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Active Recombinant Human IL15 Protein, His-tagged

Cat.No. : IL15-004H
Product Overview : Recombinant human IL-15 protein (49-162 aa), fused to His-tag was expressed in E.coli and purified by using conventional chromatography after refolding of the isolated inclusion bodies in a redox buffer
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Description : Interleukin (IL)-15 is a pleiotropic cytokine that plays a pivotal role in both innate and adaptive immunity. IL-15 is unique among cytokines due to its participation in a trans signaling mechanism in which IL-15 receptor alpha (IL-15R alpha) from one subset of cells presents IL-15 to neighboring IL-2R beta/gamma(c)-expressing cells. This newly discovered cytokine is produced by a wide variety of cells and tissues and like IL-2, stimulates the proliferation of T-lymphocytes and induces the generation of cytotoxic T-lymphocytes (CTLs) and lymphokine-activated killer (LAK) cells.
Source : E. coli
Species : Human
Tag : His
Form : Liquid
Bio-activity : Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 for this effect is less or equal to 1 ng/mL.
Molecular Mass : 13.9 kDa (123aa) confirmed by MALDI-TOF
AA Sequence : MNWVNVISDLKKIEDLIQSMHIDAT LYTESDVHPSCKVTAMKCFLLELQV ISLESGDASIHDTVENLIILANNSL SSNGNVTESGCKECEELEEKNIKEF LQSFVHIVQMFINTSLEHHHHHH
Endotoxin : < 1.0 EU per 1μg of protein (determined by LAL method)
Purity : > 95% by SDS-PAGE
Notes : For research use only. This product is not intended or approved for human, diagnostics or veterin.
Storage : Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70 centigrade.
Avoid repeated freezing and thawing cycles.
Concentration : 1 mg/mL (determined by Bradford assay)
Storage Buffer : In Phosphate Buffer Saline(pH7.4) containing 10% glycerol.
Gene Name : IL15 interleukin 15 [ Homo sapiens (human) ]
Official Symbol : IL15
Synonyms : Interleukin-15; IL-15; interleukin-15
Gene ID : 3600
mRNA Refseq : NM_172175
Protein Refseq : NP_751914
MIM : 600554
UniProt ID : P40933

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What techniques differentiate IL15 and IL2 receptor binding kinetics? 10/22/2022

Techniques discern IL15 and IL2 receptor binding kinetics.

What experimental methods study IL15's involvement in autoimmune diseases? 06/23/2022

Methods explore IL15's role in autoimmune disease mechanisms.

What are the applications of IL15 knockout models in deciphering its functions? 04/26/2022

IL15 knockout models provide insights into its diverse functions.

How do you analyze IL15's impact on memory T cell differentiation? 09/19/2021

IL15's influence on memory T cell differentiation investigated.

How does immunofluorescence microscopy visualize IL15 localization in cells? 07/26/2021

Immunofluorescence microscopy visualizes IL15 subcellular localization.

What approaches validate IL15's role in inflammatory responses? 02/04/2021

Approaches validate IL15's involvement in inflammatory responses.

Could you explain the role of IL15 in NK cell cytotoxicity assays? 10/26/2019

IL15's role in NK cell cytotoxicity analyzed using assays.

Can you detail the use of ELISA in quantifying IL15 secretion? 02/03/2018

ELISA measures IL15 secretion accurately.

How is IL15's effect on regulatory T cells quantified using qPCR? 06/08/2017

qPCR quantifies IL15's impact on regulatory T cells.

How does flow cytometry assess IL15-induced T-cell proliferation? 05/26/2017

Flow cytometry quantifies IL15-driven T-cell proliferation.

Customer Reviews (3)

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Reviews
04/19/2018

    Mapping protein- interactions in DNA transcription for gene regulation.

    02/20/2018

      Suitable for cytotoxicity assays.

      10/22/2017

        Effective in NK cell activation.

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