Active Recombinant Human IL15 Protein, His-tagged
Cat.No. : | IL15-004H |
Product Overview : | Recombinant human IL-15 protein (49-162 aa), fused to His-tag was expressed in E.coli and purified by using conventional chromatography after refolding of the isolated inclusion bodies in a redox buffer |
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- Gene Information
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Description : | Interleukin (IL)-15 is a pleiotropic cytokine that plays a pivotal role in both innate and adaptive immunity. IL-15 is unique among cytokines due to its participation in a trans signaling mechanism in which IL-15 receptor alpha (IL-15R alpha) from one subset of cells presents IL-15 to neighboring IL-2R beta/gamma(c)-expressing cells. This newly discovered cytokine is produced by a wide variety of cells and tissues and like IL-2, stimulates the proliferation of T-lymphocytes and induces the generation of cytotoxic T-lymphocytes (CTLs) and lymphokine-activated killer (LAK) cells. |
Source : | E. coli |
Species : | Human |
Tag : | His |
Form : | Liquid |
Bio-activity : | Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 for this effect is less or equal to 1 ng/mL. |
Molecular Mass : | 13.9 kDa (123aa) confirmed by MALDI-TOF |
AA Sequence : | MNWVNVISDLKKIEDLIQSMHIDAT LYTESDVHPSCKVTAMKCFLLELQV ISLESGDASIHDTVENLIILANNSL SSNGNVTESGCKECEELEEKNIKEF LQSFVHIVQMFINTSLEHHHHHH |
Endotoxin : | < 1.0 EU per 1μg of protein (determined by LAL method) |
Purity : | > 95% by SDS-PAGE |
Notes : | For research use only. This product is not intended or approved for human, diagnostics or veterin. |
Storage : | Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70 centigrade. Avoid repeated freezing and thawing cycles. |
Concentration : | 1 mg/mL (determined by Bradford assay) |
Storage Buffer : | In Phosphate Buffer Saline(pH7.4) containing 10% glycerol. |
Gene Name : | IL15 interleukin 15 [ Homo sapiens (human) ] |
Official Symbol : | IL15 |
Synonyms : | Interleukin-15; IL-15; interleukin-15 |
Gene ID : | 3600 |
mRNA Refseq : | NM_172175 |
Protein Refseq : | NP_751914 |
MIM : | 600554 |
UniProt ID : | P40933 |
Products Types
◆ Lysates | ||
IL15-5248HCL | Recombinant Human IL15 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionTechniques discern IL15 and IL2 receptor binding kinetics.
Methods explore IL15's role in autoimmune disease mechanisms.
IL15 knockout models provide insights into its diverse functions.
IL15's influence on memory T cell differentiation investigated.
Immunofluorescence microscopy visualizes IL15 subcellular localization.
Approaches validate IL15's involvement in inflammatory responses.
IL15's role in NK cell cytotoxicity analyzed using assays.
ELISA measures IL15 secretion accurately.
qPCR quantifies IL15's impact on regulatory T cells.
Flow cytometry quantifies IL15-driven T-cell proliferation.
Customer Reviews (3)
Write a reviewMapping protein- interactions in DNA transcription for gene regulation.
Suitable for cytotoxicity assays.
Effective in NK cell activation.
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