Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Active Recombinant Human EBI3&IL12A, MIgG2a Fc-tagged

Cat.No. : EBI3&IL12A-122H
Product Overview : The human IL-35 complex composed of the Ebi3 subunit(NP_005746.2) (Arg21-Lys229) and the IL-12a(p35) subunit (NP_000873.2)(Arg57-Ser253) is fused through a polypeptide linker to the Fc portion of mouse IgG2awas expressed in CHO cell
  • Specification
  • Gene Information
  • Related Products
Description : The gene EBI3 was identified by its induced expression in B lymphocytes in response Epstein-Barr virus infection. It encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form interleukin 27 (IL-27). IL-27 regulates T cell and inflammatory responses, in part by activating the Jak/STAT pathway of CD4+ T cells, while the other gene IL12A encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.
Source : CHO cells
Species : Human
Tag : MIgG2a Fc-tagged
Form : Lyophilized from 0.2μm-filtered solution in PBS.
Bio-activity : Bioactivity was measured in a cell proliferation assay using anti-CD3 activated human peripheral mononuclear cells.
Molecular Mass : 75 KDa (monomer)
AA Sequence : The human IL-35 complex composed of the Ebi3 subunit(NP_005746.2) (Arg21-Lys229) and the IL-12a(p35) subunit (NP_000873.2)(Arg57-Ser253) is fused through a polypeptide linker to the Fc portion of mouse IgG2a.
Endotoxin : <0.06 eu/μg as determined by lal test.>
Purity : >98%, by SDS-PAGE under reducing conditions.
Stability : Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Reconstitution : Reconstitute at 100μg/ml in sterile PBS.
Warning : Avoid freeze/thaw cycles.
Gene Name : EBI3; IL12A
Official Symbol : IL35
Synonyms : P35; CLMF; NFSK; NKSF1; IL-12A;IL27B; IL-27B; EBI3; Epstein-Barr virus induced 3
Gene ID : 10148; 3592
mRNA Refseq : NM_000882& NM_005755
Protein Refseq : NP_005746& NP_000873
MIM : 161560& 605816
UniProt ID : Q14213&P29459
Chromosome Location : 3q25.33&19p13.3

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (6)

Ask a question
What is the dosage of IL35? 11/03/2022

The dosage of IL35 is usually determined in clinical trials and adjusted according to the specific situation of the patient.

How is the regulatory effect of IL35 on immune cells realized? 07/15/2022

The IL35 works by regulating the function and activity of immune cells, thereby influencing the immune response of the immune system.

What is the effect of recombinant IL35 on the immune system? 03/14/2022

Recombinant IL35 can inhibit the activity of the immune system, thereby regulating the inflammatory response and the function of immune cells.

Can recombinant IL35 be used with other drugs? 08/11/2021

Combination use of IL35 needs to be evaluated in clinical practice to determine possible interactions and safety.

How to evaluate the efficacy of IL35? 10/06/2020

The efficacy of IL35 is typically evaluated by biomarkers in clinical trials, degree of disease remission, and patient feedback.

Can recombinant IL35 treat autoimmune diseases? 01/20/2020

IL35 shows potential to treat certain autoimmune diseases, but further research is needed to determine its effectiveness and safety.

Customer Reviews (3)

Write a review
Reviews
11/23/2021

    IL34 can maintain its biological activity and structural integrity under specific experimental conditions.

    11/17/2021

      IL35 has high chemical and physical stability and is suitable for long-term storage and use.

      12/20/2019

        Products undergo strict quality control to ensure the consistency and stability of each batch.

        Ask a Question for All IL35 Products

        Required fields are marked with *

        My Review for All IL35 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends