"GFP" Related Products

Recombinant Aequorea victoria GFP

Cat.No.: GFP-11A
Product Overview: Recombinant GFP protein was expressed in E.coli and purified by using conventional chromatography.
Description: GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
Source: E. coli
Species: Aequorea victoria
Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Bio-activity: 1. Prepare a 150ul recombinant GFP with various concentrations (0.00074 nM - 6.21uM) in assay buffer and equilibrate to 25C. (Assay buffer: 10 mM Tris-HCl (pH 8.0), 10 mM EDTA, 0.02% sodium azide.)2. Read at excitation wavelengths 485nm and emission 535nm. - 96 Well Polystyrene Microplate, black (greiner bio-one) - Fluorescent plate reader (PerkinEmerl, VICTOR X3)
Molecular Mass: 26.8 kDa (238aa), confirmed by MALDI-TOF.
Protein length: 238aa
AA Sequence: MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPE GYVQERTIFF KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV YIMADKQKNG IKVNFKIRHN IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGIT HGMDELYK
Endotoxin: < 1.0 eu per 1 microgram of protein (determined by lal
Purity: >95% by SDS - PAGE
Applications: SDS-PAGE
Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
Concentration: 1 mg/ml (determined by Bradford assay)

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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