Recombinant Porcine Interleukin 4
Cat.No. : | IL4-84P |
Product Overview : | Recombinant Porcine IL-4 produced inE.Coliis a single, non-glycosylated polypeptide chain containing 110 amino acids and having a molecular mass of 12615 Dalton. The IL-4 is purified by proprietary chromatographic techniques. |
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Cat. No. : | IL4-84P |
Description : | IL4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. |
Source : | Escherichia Coli. |
Amino Acid Sequence : | The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Ly-Lys-Asp. |
Physical Appearance : | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity : | Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Formulation : | Lyophilized from a concentrated (1 mg/ml) solution in water containing no additives. |
Solubility : | It is recommended to reconstitute the lyophilized Interleikin-4 in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
Specific Activity : | The ED50 range= 1 to 4ng/ml. The biological activity is determined by measuring the dose dependent proliferation of human TF-1 cells. A concentration range of 0.1 to 10.0ng/ml is effective for most in vitro applications. |
Protein Content : | Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.218 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard. |
Stability : | Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution IL4 should be stored at 4℃ between 2-7 days and for future use below -18℃. Please prevent freeze-thaw cycles. |
Gene Name : | IL4 interleukin 4 [ Sus scrofa ] |
Synonyms : | interleukin 4; IL4 |
Gene ID : | 397225 |
mRNA Refseq : | NM_214123 |
Protein Refseq : | NP_999288 |
UniProt ID : | Q04745 |
Chromosome Location : | 2 |
Pathway : | Allograft rejection; Asthma; Autoimmune thyroid disease; Cytokine-cytokine receptor interaction; Fc epsilon RI signaling pathway; Hematopoietic cell lineage; Jak-STAT signaling pathway; T cell receptor signaling pathway |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Ask a questionCustomer Reviews (3)
Write a reviewBy testing immunogenicity in animal models, detect whether an immune response is induced.
The structure of the recombinant protein was analyzed by nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy, and the results showed that the protein was stable.
This protein has good stability, and the test results of heat resistance, acid and alkali resistance and protease degradation resistance are good.
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