Ni Agarose high flow
Cat.No. : | Ni-001A |
Product Overview : | Ni Agarose is an 4% cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin the excellent choice for high performance purification of polyhistidine-tagged proteins. |
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Cat.no : | Ni-001A |
Characteristics : | Ni Agarose High Flow charged with nickel ions will selectively retain proteins containing a histidine-tag. Histidinetagged protein is then eluted using buffers containing imidazole. For purification of insoluble histidine-tagged proteins (fusion proteins expressed as inclusion bodies) requiring denaturing conditions, up to 8 M urea or 6 M guanidine hydrochloride can be used in the buffers to solubilise insoluble proteins. |
Size : | 10 mL, 25 mL, 500 mL |
Matrix : | 4% cross-linked agarose |
Average particle size : | 90 μm |
Ligand : | Ni2+ |
Dynamic binding capacity : | 20 mg of 6xHis-tagged protein (27 kDa) /ml settled resin |
Recommended column height : | 5‐20cm |
Temperature Stability : | 4‐40 °C |
Products Types
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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