Active Recombinant Human FLT3L, His-tagged
Cat.No. : | FLT3L-20H |
Product Overview : | Recombinant Human FLT3L was purified from the endosperm tissue of barley grain (Hordeum vulgare). |
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- Gene Information
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Description : | Flt3-ligand (FMS related tyrosine kinase 3 ligand, flk-2 ligand) is involved in proliferation and differentiation of early hematopietic cells. Flt3-ligand synergizes with other CSF"s and interleukins to induce proliferation of early hematopoietic cells, but does not stimulate growth or differentation alone. Flt3-ligand binds to cells expressing the tyrosine kinase receptor Flt3. Multiple isoforms of Flt3-ligand have been identified but the predominant biologically active isoform is a transmembrane protein which can be proteolytically cleaved to generate a biologically active soluble form. |
Source : | Barley grain (Hordeum vulgare) |
Species : | Human |
Tag : | His |
Form : | PBS pH 7.2, sterile filtered |
Bio-activity : | Recombinant human Flt-3 ligand was determined by its dose-dependent effects on the proliferation of AML5 cells. The ED50 value is typically< 0.5 ng/ml corresponding to specific activity>2 x 10e6 U/mg. Optimal concentration should be determined for specific applications and cell lines. |
Molecular Mass : | Recombinant human Flt3-ligand contains 156 amino acids and a 16 a.a. Histidine-based tag for a total length of 172 a.a. and has a predicted molecular mass of 19.9 kDa. As a result of glycosylation, the recombinant protein migrates as two bands with an apparent molecular mass of 20 and 22 kDa in SDS-PAGE. |
Endotoxin : | Endotoxin level is less than 0.005ng per μg of product (0.05EU/μg). |
Purity : | Greater than 95% by SDS-PAGE gel analysis. |
Storage : | The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. |
Reconstitution : | Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile phosphate-buffered saline to a concentration of no less than 100 μg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA). |
Products Types
◆ Lysates | ||
FLT3LG-1387MCL | Recombinant Mouse FLT3LG cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionFLT3L promotes the differentiation of hematopoietic progenitor cells into various blood cell lineages, contributing to the diversity of blood cell types.
Common side effects may include flu-like symptoms, fatigue, and gastrointestinal disturbances, but the severity varies among individuals.
FLT3L is a crucial factor in the development and maturation of dendritic cells, playing a role in immune response regulation.
FLT3L supports the maintenance and self-renewal of hematopoietic stem cells in the bone marrow, influencing the overall balance of blood cell production.
Yes, elevated FLT3L levels can serve as a prognostic marker, helping to predict the likelihood of relapse and overall survival in leukemia patients.
Customer Reviews (3)
Write a reviewI am confident in the manufacturer's ability to deliver outstanding technical support.
By incorporating the FLT3L protein into my experiments, I am confident that I am utilizing a product that meets the highest standards of quality and performance.
Its high purity and integrity provide the necessary assurance for consistent and reliable results, enabling me to achieve precise and meaningful outcomes in my research.
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