Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Active Recombinant Human FLT3L, His-tagged

Cat.No. : FLT3L-20H
Product Overview : Recombinant Human FLT3L was purified from the endosperm tissue of barley grain (Hordeum vulgare).
  • Specification
  • Gene Information
  • Related Products
Description : Flt3-ligand (FMS related tyrosine kinase 3 ligand, flk-2 ligand) is involved in proliferation and differentiation of early hematopietic cells. Flt3-ligand synergizes with other CSF"s and interleukins to induce proliferation of early hematopoietic cells, but does not stimulate growth or differentation alone. Flt3-ligand binds to cells expressing the tyrosine kinase receptor Flt3. Multiple isoforms of Flt3-ligand have been identified but the predominant biologically active isoform is a transmembrane protein which can be proteolytically cleaved to generate a biologically active soluble form.
Source : Barley grain (Hordeum vulgare)
Species : Human
Tag : His
Form : PBS pH 7.2, sterile filtered
Bio-activity : Recombinant human Flt-3 ligand was determined by its dose-dependent effects on the proliferation of AML5 cells. The ED50 value is typically< 0.5 ng/ml corresponding to specific activity>2 x 10e6 U/mg. Optimal concentration should be determined for specific applications and cell lines.
Molecular Mass : Recombinant human Flt3-ligand contains 156 amino acids and a 16 a.a. Histidine-based tag for a total length of 172 a.a. and has a predicted molecular mass of 19.9 kDa. As a result of glycosylation, the recombinant protein migrates as two bands with an apparent molecular mass of 20 and 22 kDa in SDS-PAGE.
Endotoxin : Endotoxin level is less than 0.005ng per μg of product (0.05EU/μg).
Purity : Greater than 95% by SDS-PAGE gel analysis.
Storage : The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Reconstitution : Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile phosphate-buffered saline to a concentration of no less than 100 μg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA).

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (5)

Ask a question
In what ways does FLT3L impact the differentiation of hematopoietic progenitor cells? 02/21/2023

FLT3L promotes the differentiation of hematopoietic progenitor cells into various blood cell lineages, contributing to the diversity of blood cell types.

Are there any side effects associated with FLT3L-based therapies? 10/07/2022

Common side effects may include flu-like symptoms, fatigue, and gastrointestinal disturbances, but the severity varies among individuals.

How does FLT3L influence dendritic cell development and function? 06/13/2022

FLT3L is a crucial factor in the development and maturation of dendritic cells, playing a role in immune response regulation.

How does FLT3L contribute to the maintenance of hematopoietic stem cells in the bone marrow? 06/30/2021

FLT3L supports the maintenance and self-renewal of hematopoietic stem cells in the bone marrow, influencing the overall balance of blood cell production.

Can FLT3L be used as a prognostic marker in leukemia patients? 05/22/2017

Yes, elevated FLT3L levels can serve as a prognostic marker, helping to predict the likelihood of relapse and overall survival in leukemia patients.

Customer Reviews (3)

Write a review
Reviews
01/23/2023

    I am confident in the manufacturer's ability to deliver outstanding technical support.

    04/04/2021

      By incorporating the FLT3L protein into my experiments, I am confident that I am utilizing a product that meets the highest standards of quality and performance.

      08/28/2018

        Its high purity and integrity provide the necessary assurance for consistent and reliable results, enabling me to achieve precise and meaningful outcomes in my research.

        Ask a Question for All FLT3L Products

        Required fields are marked with *

        My Review for All FLT3L Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends