Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Recombinant Proteins > Recombinant Human FGF7, His-tagged

Recombinant Human FGF7, His-tagged

   Share
"FGF7" Related Products.
Cat.No.:
FGF7-29H
Product Overview:
Recombinant Human FGF7 was produced in the endosperm tissue of barley grain (Hordeum vulgare).
Description:
Keratinocyte Growth Factor (KGF, FGF-7, HBGF-7) is a member of the FGF family of 23 related mitogenic proteins. These proteins play a central role during embryonic development and postnatal growth and tissue repair, by promoting cellular proliferation and differentiation. KGF specifically stimulates epithelial cells and keratinocytes, it is the most potent growth factor identified thus far for skin keratinocytes. KGF takes part in the development of kidney and lung, angiogenesis and has a key role in wound healing following skin injuries. It shows considerable species cross-reactivity (mouse, monkey, porcine cells) as the other members of the FGF family.
Source:
Barley grain (Hordeum vulgare)
Species:
Human
Tag:
His
Form:
Sterile filtered through a 0.2 μm filter. Lyophilized from PBS, pH 7.2
Bio-activity:
Recombinant human KGF was determined by its dose dependent effect on proliferation of 4MBr5 rhesus monkey epithelial cells. The ED50 for this effect is typically below 4 ng/ml corresponding to specific activity of >2.5 x 10e5 U/mg. Optimal concentration should be determined for specific applications and cell lines.
Molecular Mass:
Recombinant human KGF contains 164 amino acids and a 16 a.a. histidine-based tag for a total length of 180 a.a. and has a predicted molecular mass of 21.2 kDa including his-tag. As a result of glycosylation, the recombinant protein migrates as two bands with an apparent molecular mass of 26 and 28 kDa in SDS-PAGE.
Endotoxin:
Endotoxin level is less than 0.005ng per μg product (0.05EU/μg).
Purity:
Greater than 95% by SDS-PAGE gel analysis.
Storage:
The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Reconstitution:
Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call
+1-631-559-9269.

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2017 Creative Biomart. All Rights Reserved.