Active Recombinant Human CSF1, His-tagged
Cat.No. : | CSF1-32H |
Product Overview : | Recombinant Human CSF1 was produced in the endosperm tissue of barley grain (Hordeum vulgare). |
- Specification
- Gene Information
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Description : | M-CSF (macrophage colony stimulating factor, colony stimulating factor 1, CSF-1) is a hemtopoietic growth factor produced by monocytes, granulocytes, endothelial cells, fibroblasts and activated B-cells and T-cells. M-CSF stimulates macrophage colony formation and regulates proliferation and differentiation of hematopoeitic stem cells into macrophages and regulates survival and differentiation of monocytes. It enhances antibody-dependent cell-mediated cytotoxity, enhances marophage killing of tumor cells and microorganisms, regulates the release of cytokines and inhibits bone resorption by osteoclasts. Human M-CSF shows biological ativity on murine and rat cells and although murine M-CSF is active in rat cells it is inactive in human cells. |
Source : | Barley grain (Hordeum vulgare) |
Species : | Human |
Tag : | His |
Form : | Sterile filtered through a 0,2 μm filter. Lyophilized from PBS pH 7.2 |
Bio-activity : | Recombinant human M-CSF was determined by its dose-dependent effect on the proliferation of M-NFS-60 cells. The ED50 value for this effect is typically< 2 ng/ml corresponding to specific activity>5 x 10e5 U/mg. Optimal concentration should be determined for specific applications and cell lines. |
Molecular Mass : | Recombinant human M-CSF contains 159 amino acids and a 16 a.a. Histidine-based tag for a total length of 175 a.a. and has a predicted molecular mass of 20.7 kDa. The recombinant protein migrates with an apparent molecular mass of 24-26 kDa in SDS-PAGE. |
Endotoxin : | Endotoxin level is less than 0.005ng per μg of product (0.05EU/μg). |
Purity : | Greater than 95% by SDS-PAGE gel analysis. |
Storage : | The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. |
Reconstitution : | Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. The solution can be further diluted into aliquots in aquous buffers. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). |
Gene Name : | CSF1 colony stimulating factor 1 (macrophage) [ Homo sapiens ] |
Official Symbol : | CSF1 |
Synonyms : | CSF1; colony stimulating factor 1 (macrophage); macrophage colony-stimulating factor 1; M CSF; MCSF; MGC31930; lanimostim; CSF-1; |
Gene ID : | 1435 |
mRNA Refseq : | NM_000757 |
Protein Refseq : | NP_000748 |
MIM : | 120420 |
UniProt ID : | P09603 |
Chromosome Location : | 1p21-p13 |
Pathway : | Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Cytokines and Inflammatory Response, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Integrins in angiogenesis, organism-specific biosystem; Osteoclast differentiation, organism-specific biosystem; |
Function : | cytokine activity; growth factor activity; growth factor activity; macrophage colony-stimulating factor receptor binding; macrophage colony-stimulating factor receptor binding; protein homodimerization activity; |
Products Types
◆ Recombinant Protein | ||
Csf1-2161R | Recombinant Rat Csf1 Protein, His&GST-tagged | +Inquiry |
CSF1-1962H | Recombinant Human CSF1 Protein, GST-tagged | +Inquiry |
CSF1-335C | Active Recombinant Human CSF1 Protein (159 aa) | +Inquiry |
Csf1-46M | Recombinant Active Mouse CSF1 Protein, His-tagged(C-ter) | +Inquiry |
CSF1-414M | Active Recombinant Mouse CSF1 protein(Met1-Glu262), His-tagged | +Inquiry |
◆ Lysates | ||
CSF1-001MCL | Recombinant Mouse CSF1 cell lysate | +Inquiry |
CSF1-1934HCL | Recombinant Human CSF1 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionCSF1 is implicated in tumor-associated macrophages, influencing tumor growth, invasion, and the tumor microenvironment.
Side effects may include immune-related adverse events, inflammation, and alterations in white blood cell counts.
Yes, there is ongoing research on combining CSF1-targeted therapies with other immunotherapies or conventional treatments to enhance efficacy.
Challenges include maintaining the balance of immune responses, potential off-target effects, and variability in patient responses.
Studies are exploring its role in neuroinflammation, neuroprotection, and potential therapeutic interventions for conditions like Alzheimer's disease.
Customer Reviews (3)
Write a reviewThe CSF1 protein is renowned for its exceptional quality and reliability, making it an ideal choice to fulfill my experimental requirements.
With its high purity and stability, this protein ensures dependable and accurate results, which are crucial for the success of my research endeavors.
Whether it is for investigating cellular pathways, studying protein functions, or exploring signaling cascades, the CSF1 protein offers unparalleled versatility and performance.
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