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Active Recombinant Human KITLG, His-tagged

Cat.No. : KITLG-33H
Product Overview : Recombinant Human SCF was produced in the endosperm tissue of barley grain (Hordeum vulgare).
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Description : Stem Cell Factor (SCF, also known as c-kit ligand, mast cell growth factor and steel factor) is a hematopoietic growth factor acting at the early stages of hematopoiesis. SCF promotes the survival, proliferation and differentiation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also stimulates the growth and activation of mast cells and eosinophils. SCF has been shown to act synergistically with other growth factors, such as GM-CSF, G-CSF, IL-7 and EPO. Human SCF shows low activity on murine cells, while murine and rat SCF are fully active on human cells.
Source : Barley grain (Hordeum vulgare)
Species : Human
Tag : His
Form : Sterile filtered through a 0.2 μm filter. Lyophilized from PBS, pH 7.2
Bio-activity : Recombinant human SCF is measured by its dose-dependent effect on the proliferation of TF-1 cells. The ED50 for this effect is typically between 2 - 10 ng/ml corresponding to specific activity of 1 – 5 x 10e5 U/mg. We recommend that the optimal concentration for each specific application be determined by an initial dose-response assay.
Molecular Mass : Recombinant human SCF contains 165 amino acids and a 16 a.a. histidine-based tag for a total length of 181 a.a. and has a predicted molecular mass of 20.7 kDa including his-tag. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 22-24 kDa in SDS-PAGE.
Endotoxin : Endotoxin level is less than 0.005ng per μg of product (0.05EU/μg)
Purity : Greater than 95% by SDS-PAGE gel analysis
Storage : The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Reconstitution : Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Gene Name : KITLG KIT ligand [ Homo sapiens ]
Official Symbol : KITLG
Synonyms : KITLG; KIT ligand; MGF; kit ligand; familial progressive hyperpigmentation 2; FPH2; Kitl; KL 1; mast cell growth factor; SCF; SF; steel factor; stem cell factor; c-Kit ligand; KL-1; SHEP7; kit-ligand; DKFZp686F2250;
Gene ID : 4254
mRNA Refseq : NM_000899
Protein Refseq : NP_000890
MIM : 184745
UniProt ID : P21583
Chromosome Location : 12q22
Pathway : C-MYB transcription factor network, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Kit Receptor Signaling Pathway, organism-specific biosystem; Melanogenesis, organism-specific biosystem;
Function : cytokine activity; growth factor activity; protein binding; stem cell factor receptor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How does KITLG influence stem cell factor (SCF) signaling pathways? 11/10/2022

Overexpression or mutations in KITLG and its receptor have been implicated in various cancers, including gastrointestinal stromal tumors (GISTs), mastocytosis, and certain leukemias.

What are the implications of KITLG in oncogenesis and tumor progression? 07/20/2022

Given its role in cell proliferation and differentiation, KITLG has been explored as a therapeutic agent in stem cell transplantation settings, and its pathway is a target in certain cancer treatments.

What role does KITLG play in melanogenesis and pigmentation? 01/17/2022

Dysregulation or mutations in KITLG have been associated with certain disorders like piebaldism, a genetic condition characterized by unpigmented patches of skin and hair.

Are there any known disorders associated with mutations or dysregulation of KITLG? 06/22/2020

Upon binding to c-KIT, KITLG activates the SCF signaling pathways, which include the MAPK, PI3K, and JAK/STAT pathways, influencing cell survival, proliferation, and differentiation.

What is the primary biological function of KITLG in the hematopoietic system? 04/12/2020

KITLG binds to the c-KIT receptor, a tyrosine kinase receptor, leading to its dimerization and autophosphorylation, which activates downstream signaling pathways.

How has KITLG been explored as a therapeutic target or agent in clinical settings? 02/10/2020

Given its role in cell proliferation and differentiation, KITLG has been explored as a therapeutic agent in stem cell transplantation settings, and its pathway is a target in certain cancer treatments.

How does KITLG interact with its receptor, c-KIT? 12/21/2019

KITLG and its receptor c-KIT are involved in melanogenesis. Their interaction regulates the proliferation and migration of melanocytes, influencing skin, hair, and eye pigmentation.

Customer Reviews (3)

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Reviews
01/23/2022

    Reliable for immunoprecipitation assay.

    03/01/2021

      No contamination, very clean.

      02/09/2020

        Worked perfectly in Western.

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