Recombinant Human AMBP cell lysate
Cat.No. : | AMBP-001HCL |
Product Overview : | Human AMBP / Alpha 1 microglobulin derived in Human cells. The whole cell lysate is provided in 1X Sample Buffer. |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | AMBP alpha-1-microglobulin/bikunin precursor [ Homo sapiens ] |
Official Symbol : | AMBP |
Synonyms : | AMBP; alpha-1-microglobulin/bikunin precursor; ITI, ITIL; protein AMBP; bikunin; complex forming glycoprotein heterogeneous in charge; EDC1; growth inhibiting protein 19; HCP; HI30; IATIL; inter alpha trypsin inhibitor light chain; ITILC; protein HC; trypstatin; uristatin; uronic acid rich protein; UTI; uronic-acid-rich protein; growth-inhibiting protein 19; inter-alpha-trypsin inhibitor light chain; complex-forming glycoprotein heterogeneous in charge; A1M; ITI; ITIL; |
Gene ID : | 259 |
mRNA Refseq : | NM_001633 |
Protein Refseq : | NP_001624 |
MIM : | 176870 |
UniProt ID : | P02760 |
Chromosome Location : | 9q32-q33 |
Function : | IgA binding; calcium channel inhibitor activity; calcium oxalate binding; heme binding; peptidase inhibitor activity; protein homodimerization activity; serine-type endopeptidase inhibitor activity; transporter activity; |
Products Types
◆ Recombinant Protein | ||
AMBP-331H | Recombinant Human AMBP Protein, His (Fc)-Avi-tagged | +Inquiry |
AMBP-302R | Recombinant Rat AMBP Protein, His (Fc)-Avi-tagged | +Inquiry |
AMBP-128H | Recombinant Human AMBP Protein, His-tagged | +Inquiry |
AMBP-129C | Recombinant Cattle AMBP Protein, His-tagged | +Inquiry |
Ambp-590M | Recombinant Mouse Ambp Protein, MYC/DDK-tagged | +Inquiry |
◆ Native Protein | ||
AMBP-5312H | Native Human Alpha-1-Microglobulin/Bikunin Precursor | +Inquiry |
AMBP-27H | Native Human AMBP | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionAMBP expression levels and isoform variations can serve as diagnostic biomarkers for inflammatory diseases, immune dysregulation, or infection, aiding in disease diagnosis, monitoring, and prognostication.
AMBP expression is regulated by genetic and epigenetic factors, including transcription factors, DNA methylation, and histone modifications, which influence its expression patterns in different cell types and disease contexts.
AMBP modulates immune cell activities by regulating cellular adhesion, migration, phagocytosis, cytokine production, and immune cell differentiation through intracellular signaling pathways, such as MAPK and NF-魏B pathways.
AMBP participates in the regulation of complement activation by interacting with complement components, controlling the formation of membrane attack complexes and modulating the inflammatory response.
Targeting AMBP for therapeutic interventions in immune-mediated disorders or inflammatory conditions can be achieved through approaches such as small molecule inhibitors, antibody-based therapies, or gene editing techniques.
AMBP plays important roles in the immune system, including regulating inflammatory responses, modulating complement activation, and contributing to host defense mechanisms.
AMBP is released or secreted by immune cells through various mechanisms, including vesicular trafficking, exosomes, or proteolytic cleavage, which can be exploited for therapeutic purposes or biomarker discovery.
AMBP contributes to tissue homeostasis and immune surveillance in different organs, such as the kidney, lung, or reproductive system, and its dysregulation can have implications in tissue damage, autoimmune diseases, or infections in specific organ systems.
AMBP interacts with various immune-related proteins, such as complement components C3 and C5, cytokines, and cell surface receptors, influencing immune cell functions, immune signaling, and inflammation processes.
The structural features of AMBP, including its two domains (伪1-microglobulin and bikunin), are crucial for its immune-related functions, protein-protein interactions, and stability.
Customer Reviews (3)
Write a reviewCompatibility with various protein modification detection methods.
Enhanced data reproducibility in protein quantification.
Comprehensive profiling of protein expression patterns.
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