Recombinant Human CD1B & B2M cell lysate

• Cat.No.: CD1B & B2M-001HCL
• Product Overview: Human CD1B & B2M Heterodimer derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.

Specification

• Source: Human cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: CD1B CD1b molecule [ Homo sapiens ]; B2M beta-2-microglobulin [ Homo sapiens ]
• Official Symbol: CD1B; B2M
• Synonyms: R1; CD1; CD1A; T-cell surface glycoprotein CD1b; CD1B antigen, b polypeptide; cortical thymocyte antigen CD1B; differentiation antigen CD1-alpha-3; beta-2-microglobulin; beta-2-microglobin; beta chain of MHC class I molecules
• Gene ID: 910; 567
• mRNA Refseq: NM_001764; NM_004048
• Protein Refseq: NP_001755; NP_004039
• MIM: 188360; 109700
• UniProt ID: P29016; P61769
• Chromosome Location: 1q23.1; 15q21-q22.2
• Pathway: Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem. Adaptive Immune System, organism-specific biosystem; Antigen processing and presentation, conserved biosystem
• Function: beta-2-microglobulin binding; exogenous lipid antigen binding. identical protein binding; protein binding