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Recombinant Mouse IL6ST cell lysate

Cat.No. : IL6ST-001MCL
Product Overview : Mouse IL6ST / CD130 derived in CHO cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : CHO cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Il6st interleukin 6 signal transducer [ Mus musculus ]
Official Symbol : IL6ST
Synonyms : IL6ST; interleukin 6 signal transducer; interleukin-6 receptor subunit beta; IL-6RB; IL-6R-beta; IL-6R subunit beta; membrane glycoprotein 130; IL-6 receptor subunit beta; interleukin-6 signal transducer; oncostatin-M receptor subunit alpha; CD130; gp130; AA389424; BB405851; D13Ertd699e; 5133400A03Rik;
Gene ID : 16195
mRNA Refseq : NM_010560
Protein Refseq : NP_034690
Pathway : Adipogenesis, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; ESC Pluripotency Pathways, organism-specific biosystem; IL-6 signaling Pathway, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem;
Function : ciliary neurotrophic factor receptor activity; ciliary neurotrophic factor receptor binding; growth factor binding; contributes_to interleukin-11 binding; contributes_to interleukin-11 receptor activity; interleukin-6 receptor activity; protein binding; protein heterodimerization activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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How to detect IL6ST activity? 04/26/2022

The activity of IL6ST is usually detected by cell differentiation and proliferation assay.

In what diseases does IL6ST have applications? 04/20/2021

IL6ST may be used in the future to treat pulmonary fibrosis, chronic pulmonary obstruction, rheumatoid arthritis, and other chronic kidney diseases.

Are there any effects of IL6ST on cell differentiation? 04/15/2021

IL6ST is involved in various inflammatory responses and cell differentiation processes.

What are the physical and chemical properties of IL6ST? 02/03/2021

The physical and chemical properties of IL6ST include molecular weight, voltammetry potential, structure and stability.

Why research IL6ST? 02/09/2019

The study of IL6ST helps us to better understand the disease mechanism involved in IL6ST, and provides new ideas and means for disease treatment.

What is the preparation method of IL6ST? 01/27/2019

The IL6ST is usually prepared by transforming E. coli into an expression vector and making it express the IL6ST protein.

Customer Reviews (3)

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Reviews
02/28/2022

    IL6ST proteins can be functionally enhanced or altered by protein modification techniques.

    08/04/2020

      As an immunotherapy drug, it has good effect and high safety.

      09/06/2019

        Has a good inhibitory effect on cell proliferation and survival.

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