Recombinant Canine XCL1 cell lysate
Cat.No. : | XCL1-001CCL |
Product Overview : | Canine XCL1 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer. Browse all transfected cell lysate positive controls |
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- Gene Information
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Source : | Baculovirus-Insect Cells |
Species : | Canine |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | cytokine SCM-1 beta-like [ Canis lupus familiaris (dog) ] |
Official Symbol : | XCL1 |
Synonyms : | XCL1; XCL2 |
Gene ID : | 490356 |
mRNA Refseq : | XM_547477 |
Protein Refseq : | XP_547477 |
UniProt ID : | J9NRS1 |
Chromosome Location : | chromosome: 7 |
Pathway : | Chemokine receptors bind chemokines, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem |
Products Types
◆ Recombinant Protein | ||
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XCL1-6264R | Recombinant Rat XCL1 Protein, His (Fc)-Avi-tagged | +Inquiry |
XCL1-1187C | Recombinant Cynomolgus XCL1 Protein, His-tagged | +Inquiry |
Xcl1-182M | Recombinant Mouse Xcl1 protein | +Inquiry |
◆ Lysates | ||
XCL1-452CCL | Recombinant Cynomolgus XCL1 cell lysate | +Inquiry |
XCL1-466HCL | Recombinant Human XCL1 cell lysate | +Inquiry |
XCL1-480MCL | Recombinant Mouse XCL1 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionXCL1 expression is regulated by various stimuli, including inflammatory signals. Understanding how these stimuli modulate XCL1 secretion provides insights into its dynamic role in immune activation.
XCL1 influences the differentiation and function of dendritic cells, affecting the priming and activation of T cells. Understanding these processes has implications for modulating adaptive immune responses in various contexts.
XCL1 is involved in the formation of immune synapses between dendritic cells and T cells. Molecular events include the coordinated expression of adhesion molecules and the release of other signaling molecules.
XCL1 binds to its receptor XCR1, initiating intracellular signaling cascades. This interaction influences immune cell function by directing the migration and activation of specific immune cell subsets.
XCL1 is implicated in the host immune response to viral infections. Its role in recruiting and activating immune cells may influence the outcome of infections, impacting viral clearance and immune memory.
Customer Reviews (3)
Write a reviewConsistent high quality, responsive customer service, and seamless delivery
Commendable product quality, professional customer service, and punctual delivery.
Outstanding product quality, swift delivery, and helpful customer service. Highly recommend!
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