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Recombinant Human BTK cell lysate

Cat.No. : BTK-001HCL
Product Overview : Human Bruton Tyrosine Kinase / BTK Kinase derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Baculovirus-Insect Cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : BTK Bruton agammaglobulinemia tyrosine kinase [ Homo sapiens ]
Official Symbol : BTK
Synonyms : BTK; Bruton agammaglobulinemia tyrosine kinase; AGMX1, IMD1; tyrosine-protein kinase BTK; ATK; PSCTK1; XLA; B-cell progenitor kinase; agammaglobulinaemia tyrosine kinase; tyrosine-protein kinase BTK isoform (lacking exon 14); dominant-negative kinase-deficient Brutons tyrosine kinase; AT; BPK; IMD1; AGMX1; MGC126261; MGC126262;
Gene ID : 695
mRNA Refseq : NM_000061
Protein Refseq : NP_000052
MIM : 300300
UniProt ID : Q06187
Chromosome Location : Xq21.33-q22
Pathway : Adaptive Immune System, organism-specific biosystem; Antigen Activates B Cell Receptor Leading to Generation of Second Messengers, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; B cell receptor signaling pathway, organism-specific biosystem; B cell receptor signaling pathway, conserved biosystem; BCR signaling pathway, organism-specific biosystem; Class I PI3K signaling events, organism-specific biosystem;
Function : ATP binding; ATP binding; identical protein binding; kinase activity; lipid binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; phosphatidylinositol-3,4,5-trisphosphate binding; protein binding; protein kinase activity; protein tyrosine kinase activity; protein tyrosine kinase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What is the current progress in drug development for BTK? 04/02/2022

The development of drugs targeting BTK is currently in the clinical trial stage, including small molecule inhibitors against BTK and inhibitors targeting BTK signaling pathways.

How can BTK be used as a biomarker in clinical practice? 12/28/2021

In clinical practice, the expression level of BTK can be detected to predict disease progression and prognosis, and to guide the choice of treatment regimen. For example, in the diagnosis and treatment of lymphoma and leukemia, testing the expression level of BTK can help doctors determine the type, stage and prognosis of the disease, and guide the choice of treatment regimen.

What is the significance of studying BTK proteins? 08/26/2021

BTK proteins can help to gain insight into the mechanisms of B cell development and activation, as well as the pathogenesis of certain diseases such as lymphoma and leukemia. In addition, it is of great significance for the development of new drugs and treatment strategies.

Does BTK protein play a role in tumor immunotherapy? 03/22/2021

BTK protein plays an important role in tumor immunotherapy, which can activate the immune response of tumor cells, promote apoptosis of tumor cells and inhibit the proliferation of tumor cells.

How is BTK protein related to viral infection? 08/01/2020

Studies have shown that BTK protein can participate in the regulation of antiviral immune responses and affect the course of viral infection.

What are the applications of BTK protein in drug discovery? 02/12/2020

It can be used as a target for the development of new drugs, or as a drug carrier for the directional delivery of drugs.

Customer Reviews (3)

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Reviews
02/08/2022

    High purity, lack of impurities, in the experiment performance is very efficient.

    02/03/2022

      Short half-life, more flexible dosage plan.

      09/12/2021

        It's stable in all kinds of environments.

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