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Recombinant Human AK4 cell lysate

Cat.No. : AK4-001HCL
Product Overview : Human AK4 / AK3L1 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Baculovirus-Insect Cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : AK4 adenylate kinase 4 [ Homo sapiens ]
Official Symbol : AK4
Synonyms : AK4; adenylate kinase 4; adenylate kinase 3 , adenylate kinase 3 like 1 , AK3, AK3L1; adenylate kinase isoenzyme 4, mitochondrial; adenylate kinase 3-like 1; ATP-AMP transphosphorylase; GTP:AMP phosphotransferase; mitochondrial adenylate kinase-3; nucleoside-triphosphate-adenylate kinase; AK3; AK3L1; AK3L2; MGC166959;
Gene ID : 205
mRNA Refseq : NM_001005353
Protein Refseq : NP_001005353
MIM : 103030
UniProt ID : P27144
Chromosome Location : 1p31.3
Pathway : Metabolic pathways, organism-specific biosystem; Purine metabolism, organism-specific biosystem; Purine metabolism, conserved biosystem;
Function : ATP binding; GTP binding; adenylate kinase activity; nucleotide binding; transferase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (23)

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What are the different cellular processes involving AK4 protein? 03/25/2023

AK4 is involved in various cellular processes, including energy metabolism, maintaining cellular ATP/AMP ratio, and protecting cells from oxidative stress. It also plays a role in the regulation of apoptosis and cell proliferation.

Does AK4 protein play a role in cellular respiration? 10/02/2022

AK4 protein does not directly participate in cellular respiration itself, but it contributes to energy metabolism by regulating ATP levels. ATP generated through cellular respiration is used by AK4 to regenerate ADP, ensuring an adequate supply of ATP for cellular functions. Thus, AK4 indirectly supports cellular respiration by maintaining a proper ATP/ADP balance.

Are there any animal models available to study AK4 protein's function? 08/17/2022

Yes, animal models, such as mice, have been used to study AK4 protein's function and its impact on various diseases. These models allow researchers to investigate the physiological and pathological roles of AK4 and assess its therapeutic potential.

Are there any ongoing clinical trials targeting AK4 protein? 07/08/2022

Currently, there are no known clinical trials specifically targeting AK4 protein. However, research on AK4 and its potential therapeutic implications is ongoing, and future clinical trials may explore the therapeutic relevance of AK4 modulation in diseases such as cancer and neurodegenerative disorders.

Is AK4 protein targeted by any drugs? 05/28/2022

As of now, there are no drugs specifically targeting AK4 protein. However, further research may uncover its therapeutic potential, particularly in diseases where AK4 dysregulation is observed.

How is AK4 protein regulated in the cell? 09/30/2021

AK4 protein expression and activity can be regulated at multiple levels, including transcriptional regulation, post-translational modifications, and interaction with other cellular proteins. Various signaling pathways and conditions can influence AK4 expression and activity.

Can AK4 protein levels be regulated by environmental factors? 06/14/2021

While research on the direct influence of environmental factors on AK4 protein levels is limited, it is plausible that certain environmental factors, such as diet or exposure to toxins, may indirectly affect AK4 expression or activity. However, further studies are needed to determine the specific environmental factors that may influence AK4 regulation and their impact on health and disease.

Are there any known inhibitors or activators of AK4 protein? 10/02/2020

Currently, there are no known specific inhibitors or activators of AK4 protein. The research on AK4 modulation is still in its early stages, and more investigations are needed to identify compounds that can selectively modulate AK4 activity. Such compounds could have potential therapeutic applications in diseases involving abnormal energy metabolism.

Can AK4 protein be used as a biomarker for any diseases? 09/29/2020

Recent research suggests that altered expression and activity of AK4 protein may be associated with certain diseases. For example, decreased AK4 levels have been observed in certain cancers, while increased levels have been linked to neurodegenerative disorders.

Can AK4 protein be targeted for therapeutic interventions? 05/19/2020

While specific drugs targeting AK4 protein are not currently available, further research may uncover its potential as a therapeutic target. Modulating AK4 activity or expression may have implications in diseases where its dysregulation occurs, such as cancer or neurodegenerative disorders.

How is AK4 protein linked to cancer? 02/20/2020

Research has shown that AK4 protein expression is often downregulated in various types of cancer, including lung, breast, and colon cancer. Decreased AK4 levels have been associated with tumor progression and poor prognosis. Loss of AK4 function may affect ATP regeneration and energy metabolism in cancer cells, promoting their survival and proliferation.

Does AK4 protein have any other cellular functions besides energy metabolism? 05/21/2019

Apart from its role in energy metabolism, AK4 protein has been implicated in apoptosis regulation. It has been found to interact with Bcl-2 family proteins, which are involved in the regulation of cell death. AK4 may modulate apoptosis processes by influencing the release of cytochrome c from mitochondria and the activation of caspases.

Could AK4 protein be used as a biomarker for diagnosing certain diseases? 01/27/2019

AK4 protein levels could potentially be used as a biomarker for certain diseases. For example, decreased AK4 expression has been observed in some types of cancer, and measuring AK4 levels in patient samples may help in cancer diagnosis and prognosis. However, more research is needed to establish its diagnostic utility in different diseases and determine the specificity and sensitivity of AK4 as a biomarker.

Is there any ongoing research on AK4 protein? 12/09/2018

Yes, ongoing research focuses on exploring the role of AK4 protein in different diseases, understanding its regulation and interactions, and identifying potential therapeutic interventions. Multiple studies are investigating its involvement in cancer, neurodegenerative disorders, and other conditions.

Is AK4 protein expressed in all human tissues? 11/26/2018

AK4 protein is expressed in various human tissues, but its levels may vary depending on the tissue type. High levels of AK4 expression have been observed in tissues with high energy demands, such as the brain and skeletal muscles. However, AK4 expression has also been detected in other tissues like the heart, liver, and kidney.

Are there any inhibitors or activators of AK4 protein? 02/10/2017

There are currently no specific inhibitors or activators of AK4 protein that have been extensively studied. However, some compounds and drugs have been found to potentially modulate AK4 activity, but more research is needed to fully understand their effects.

Can AK4 protein be targeted for therapeutic intervention? 02/03/2017

AK4 protein is a potential target for therapeutic intervention, particularly in diseases where dysregulated energy metabolism is implicated. Modulating AK4 activity or expression could potentially impact energy balance and cellular viability. However, the development of specific AK4-targeting therapies would require further research to fully understand its role in disease pathogenesis and to identify appropriate strategies to manipulate its function effectively and safely.

Does genetic variation in AK4 contribute to disease susceptibility? 09/02/2016

Genetic variations in AK4 have not been extensively studied with regard to disease susceptibility. However, some studies have identified single nucleotide polymorphisms (SNPs) in the AK4 gene that may be associated with certain diseases or conditions. For example, specific AK4 SNPs have been linked to increased risk of developing diabetic nephropathy, a kidney complication of diabetes. Further investigation is required to establish any direct causal relationship between AK4 genetic variations and disease susceptibility.

Can AK4 protein be targeted in cancer therapy? 12/18/2015

Targeting AK4 protein in cancer therapy holds potential, as decreased AK4 expression in certain cancers suggests a role in tumor growth and progression. Developing AK4-specific inhibitors or activators could potentially disrupt cancer cell metabolism and induce cell death or sensitize tumors to other treatments. However, extensive research is needed to understand the precise mechanisms by which AK4 contributes to tumorigenesis and to develop targeted therapies that are both effective and safe.

What are the potential clinical implications of AK4 protein? 09/15/2015

AK4 protein's involvement in energy metabolism and its association with certain diseases suggest its potential as a target for therapeutic interventions. Further understanding of its roles and regulation may enable the development of novel strategies for disease treatment or prevention.

What are the challenges in developing drugs targeting AK4 protein? 09/14/2015

One of the challenges is the lack of specific inhibitors or activators for AK4 protein. Additionally, understanding the intricate regulation and interaction networks of AK4 in different diseases is necessary to develop effective therapeutic strategies. Further research is needed to elucidate these complexities and overcome these challenges.

Are there any genetic mutations associated with AK4 protein? 05/23/2015

Currently, there are no known specific genetic mutations associated with AK4 protein. However, variations in the AK4 gene may exist and could potentially influence its expression or function. Further research is needed to determine any clinical significance of these variations.

How is AK4 protein involved in cell survival and proliferation? 01/06/2015

AK4 protein is involved in cell survival and proliferation by regulating energy metabolism. Loss of AK4 function can lead to altered ATP regeneration, affecting the energy status of cells. Reduced AK4 levels may enable cancer cells to adapt to energy stress and survive, promoting tumor growth and progression. Additionally, AK4 may influence apoptosis regulation, affecting cell survival and proliferation.

Customer Reviews (5)

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Reviews
10/31/2021

    The combined prowess of the AK4 protein and the unparalleled technical support provided by the manufacturer synergize to create an invaluable solution for my scientific needs.

    12/20/2018

      Their extraordinary level of technical support stands as a testament to their unwavering dedication to customer satisfaction.

      01/23/2016

        Its impeccable purity and reliability instill utmost confidence in its ability to generate accurate and reproducible results, catalyzing significant advancements in my research endeavors.

        07/10/2015

          The AK4 protein embodies an exceptional level of quality, rendering it a superior choice that perfectly aligns with my experimental requirements.

          02/25/2015

            the manufacturer's commitment to excellence extends beyond the mere provision of the AK4 protein.

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