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Recombinant Human CRIP2 cell lysate

Cat.No. : CRIP2-001HCL
Product Overview : Human CRIP2 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Baculovirus-Insect Cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : CRIP2 cysteine-rich protein 2 [ Homo sapiens ]
Official Symbol : CRIP2
Synonyms : CRIP2; cysteine-rich protein 2; CRP2; ESP1; CRP-2; Cystein-rich intestinal protein; CRIP;
Gene ID : 1397
mRNA Refseq : NM_001312
Protein Refseq : NP_001303
MIM : 601183
UniProt ID : P52943
Chromosome Location : 14q32.3
Function : metal ion binding; zinc ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Can CRIP2 be used as a target for drug development? 04/26/2022

The identification of CRIP2 as a potential player in various diseases raises the possibility of developing drugs that target this protein for therapeutic purposes. However, more research is needed in this area.

Can CRIP2 be targeted for therapeutic purposes? 01/08/2022

The therapeutic potential of targeting CRIP2 is an area of active research, with efforts focused on understanding its precise molecular functions and developing strategies for therapeutic intervention.

In what other diseases is CRIP2 implicated? 10/07/2020

Besides cancer, CRIP2 has been linked to inflammatory bowel diseases and cardiovascular conditions, indicating its potential relevance in various health disorders.

How does CRIP2 contribute to inflammation in the intestines? 04/17/2020

Studies suggest that CRIP2 may play a role in modulating inflammation in the intestines, making it a potential target for therapeutic interventions in inflammatory bowel diseases.

Is CRIP2 expression altered in cardiovascular diseases? 12/30/2016

Research suggests that changes in CRIP2 expression may be associated with certain cardiovascular diseases, making it a potential marker for disease progression or severity.

Customer Reviews (3)

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Reviews
03/21/2018

    Highly recommended and known for its outstanding performance in ELISA, the CRIP2 protein is a top choice for researchers in protein electron microscopy structure analysis.

    01/17/2018

      By utilizing the CRIP2 protein, researchers can confidently explore the intricate details of protein structures and gain invaluable insights into their functionality.

      02/26/2017

        Its exceptional properties and reliable results make it a valuable tool in various experimental applications.

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