Recombinant Mouse PREP cell lysate
Cat.No. : | PREP-001MCL |
Product Overview : | Mouse Prolyl endopeptidase / PREP derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Baculovirus-Insect Cells |
Species : | Mouse |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | Prep prolyl endopeptidase [ Mus musculus ] |
Official Symbol : | PREP |
Synonyms : | PREP; prolyl endopeptidase; PE; prolyl oligopeptidase; post-proline cleaving enzyme; PEP; Pop; AI047692; AI450383; D10Wsu136e; |
Gene ID : | 19072 |
mRNA Refseq : | NM_011156 |
Protein Refseq : | NP_035286 |
Function : | endopeptidase activity; hydrolase activity; peptidase activity; peptide binding; serine-type endopeptidase activity; serine-type exopeptidase activity; serine-type peptidase activity; |
Products Types
◆ Recombinant Protein | ||
PREP-2376H | Active Recombinant Human PREP Protein, His-tagged | +Inquiry |
PREP-3415R | Recombinant Rhesus Macaque PREP Protein, His (Fc)-Avi-tagged | +Inquiry |
PREP-2908H | Recombinant Human PREP Protein, MYC/DDK-tagged | +Inquiry |
PREP-4324R | Recombinant Rat PREP Protein, His (Fc)-Avi-tagged | +Inquiry |
PREP-0001H | Recombinant Human PREP Protein | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
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Q&As (6)
Ask a questionThe protein may be involved in cardiovascular disease, but the specific mechanism is not fully understood, and further research is needed to validate it.
PREP protein has been implicated in several diseases, including neurological disorders such as Alzheimer's disease, stroke, and Parkinson's disease.
Some studies suggest that PREP protein may be involved in the development of neurodegenerative diseases, but the specific mechanism and effects need to be further studied.
Studies have shown that PREP protein is abnormally expressed in some tumors, which may be related to the development and progression of tumors, but the specific role still needs to be further studied.
The role of PREP protein in digestive diseases is currently limited, and more research is needed to further explore its role and potential applications.
PREP protein can participate in the regulation of protein folding and aggregation, so it may have potential in the prevention of protein aggregation diseases.
Customer Reviews (3)
Write a reviewThe safety is very high, so you can use it with confidence.
PREP activity is maintained for a very long time and can be maintained for a long time.
It is very resistant to pollution and is not easily polluted.
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