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Recombinant Mouse PREP cell lysate

Cat.No. : PREP-001MCL
Product Overview : Mouse Prolyl endopeptidase / PREP derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Baculovirus-Insect Cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Prep prolyl endopeptidase [ Mus musculus ]
Official Symbol : PREP
Synonyms : PREP; prolyl endopeptidase; PE; prolyl oligopeptidase; post-proline cleaving enzyme; PEP; Pop; AI047692; AI450383; D10Wsu136e;
Gene ID : 19072
mRNA Refseq : NM_011156
Protein Refseq : NP_035286
Function : endopeptidase activity; hydrolase activity; peptidase activity; peptide binding; serine-type endopeptidase activity; serine-type exopeptidase activity; serine-type peptidase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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Is there an association between PREP protein and cardiovascular disease? 09/15/2022

The protein may be involved in cardiovascular disease, but the specific mechanism is not fully understood, and further research is needed to validate it.

Which diseases is PREP protein associated with? 05/28/2021

PREP protein has been implicated in several diseases, including neurological disorders such as Alzheimer's disease, stroke, and Parkinson's disease.

What is the relationship between PREP protein and the development of neurodegenerative diseases? 12/14/2020

Some studies suggest that PREP protein may be involved in the development of neurodegenerative diseases, but the specific mechanism and effects need to be further studied.

What is the role of PREP protein in tumors? 11/14/2020

Studies have shown that PREP protein is abnormally expressed in some tumors, which may be related to the development and progression of tumors, but the specific role still needs to be further studied.

What is the role of PREP protein in digestive disorders? 03/29/2020

The role of PREP protein in digestive diseases is currently limited, and more research is needed to further explore its role and potential applications.

Does PREP protein have potential in the prevention of protein aggregation diseases? 03/16/2020

PREP protein can participate in the regulation of protein folding and aggregation, so it may have potential in the prevention of protein aggregation diseases.

Customer Reviews (3)

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Reviews
01/29/2022

    The safety is very high, so you can use it with confidence.

    02/28/2021

      PREP activity is maintained for a very long time and can be maintained for a long time.

      01/21/2021

        It is very resistant to pollution and is not easily polluted.

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