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Recombinant Human RPS6KB1 cell lysate

Cat.No. : RPS6KB1-001HCL
Product Overview : Human S6K / RPS6KB1 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Baculovirus-Insect Cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : RPS6KB1 ribosomal protein S6 kinase, 70kDa, polypeptide 1 [ Homo sapiens ]
Official Symbol : RPS6KB1
Synonyms : RPS6KB1; ribosomal protein S6 kinase, 70kDa, polypeptide 1; ribosomal protein S6 kinase, 70kD, polypeptide 1 , STK14A; ribosomal protein S6 kinase beta-1; p70(S6K) alpha; PS6K; S6K1; P70S6K1; p70 S6KA; S6K-beta-1; p70 S6K-alpha; p70 S6 kinase, alpha 1; p70 S6 kinase, alpha 2; ribosomal protein S6 kinase I; serine/threonine kinase 14 alpha; serine/threonine-protein kinase 14A; 70 kDa ribosomal protein S6 kinase 1; S6K; STK14A; p70-S6K; p70-alpha; p70(S6K)-alpha;
Gene ID : 6198
mRNA Refseq : NM_003161
Protein Refseq : NP_003152
MIM : 608938
UniProt ID : P23443
Chromosome Location : 17q23.1
Pathway : Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; CDC42 signaling events, organism-specific biosystem; Cytoplasmic Ribosomal Proteins, organism-specific biosystem; ErbB signaling pathway, organism-specific biosystem;
Function : ATP binding; nucleotide binding; peptide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; ribosomal protein S6 kinase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What are the mechanisms by which RPS6KB1 modulates autophagy and cell survival? 04/14/2022

RPS6KB1 regulates autophagy and cell survival, crucial for cellular health and response to stress.

How does RPS6KB1 (Ribosomal Protein S6 Kinase B1) regulate protein synthesis and cell growth? 02/22/2021

RPS6KB1 controls protein synthesis and cell growth, playing a key role in cellular proliferation.

What is the impact of RPS6KB1 on cellular responses to stress and DNA damage? 05/22/2020

RPS6KB1 impacts cellular stress responses, including the repair of DNA damage and maintenance of genomic stability.

How does RPS6KB1 contribute to the development and progression of cancer? 01/07/2020

RPS6KB1 can contribute to cancer by promoting tumor cell growth and proliferation.

What role does RPS6KB1 play in the mTOR signaling pathway, particularly in response to nutrient availability? 08/26/2019

RPS6KB1 is a critical component of the mTOR pathway, responding to nutrient signals to regulate cell metabolism and growth.

How do alterations in RPS6KB1 expression or activity affect aging and age-related diseases? 03/05/2019

Changes in RPS6KB1 function can affect aging processes and the development of age-related diseases.

How does RPS6KB1 influence metabolic processes, especially in conditions like obesity and diabetes? 02/13/2018

RPS6KB1 influences metabolic processes, affecting how cells respond to conditions like obesity and diabetes.

Customer Reviews (3)

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Reviews
09/16/2021

    Dependable protein purification, consistently high-quality results.

    06/11/2021

      Precise mass spectrometry analysis, essential for our proteomics work.

      06/30/2019

        Efficient peptide synthesis, crucial for our peptide research projects.

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