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Recombinant Human HFE2 cell lysate

Cat.No. : HFE2-423HCL
Product Overview : Human Hemojuvelin / HFE2 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Baculovirus-Insect Cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : HFE2 hemochromatosis type 2 (juvenile) [ Homo sapiens ]
Official Symbol : HFE2
Synonyms : HFE2; hemochromatosis type 2 (juvenile); hemojuvelin; haemojuvelin; HFE2A; HJV; JH; repulsive guidance molecule c; RGMC; RGM domain family member C; hemochromatosis type 2 protein; MGC23953;
Gene ID : 148738
mRNA Refseq : NM_145277
Protein Refseq : NP_660320
MIM : 608374
UniProt ID : Q6ZVN8
Chromosome Location : 1q21.2
Pathway : Axon guidance, organism-specific biosystem; BMP receptor signaling, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Netrin-1 signaling, organism-specific biosystem;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How does HFE2 influence the body's response to anemia and hypoxia? 09/01/2022

HFE2 responds to anemia and hypoxic conditions, adjusting iron metabolism to meet physiological needs.

How do alterations in HFE2 expression or function affect the development of liver diseases and cardiomyopathy? 07/10/2021

Changes in HFE2 function can lead to liver diseases and cardiomyopathy due to abnormal iron accumulation.

What is the impact of HFE2 on the regulation of intestinal iron absorption? 04/28/2021

HFE2 impacts intestinal iron absorption, influencing how the body takes up and stores iron.

What are the mechanisms by which HFE2 modulates systemic iron homeostasis? 09/14/2020

HFE2 regulates systemic iron levels, ensuring balance between iron uptake, storage, and utilization.

What role does HFE2 play in the pathogenesis of hereditary hemochromatosis? 08/27/2018

HFE2 is involved in hereditary hemochromatosis, with its dysfunction leading to iron accumulation in organs.

How does HFE2 deficiency or mutation contribute to iron overload disorders? 06/17/2018

Mutations or deficiencies in HFE2 can cause iron overload, disrupting normal iron metabolism.

How does HFE2 (Hemojuvelin) regulate iron metabolism and hepcidin expression in the body? 02/22/2018

HFE2 is crucial in regulating iron metabolism, controlling hepcidin levels to maintain iron homeostasis.

Customer Reviews (3)

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Reviews
09/23/2022

    Efficient protein purification, delivers consistent yields and purity.

    01/10/2021

      High-quality protein crystallography services, consistently delivers exceptional results.

      06/24/2019

        Critical expertise in protein engineering, unmatched in quality and efficiency.

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