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Recombinant Mouse ACO2 cell lysate

Cat.No. : ACO2-440MCL
Product Overview : Mouse ACO2 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Baculovirus-Insect Cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Aco2 aconitase 2, mitochondrial [ Mus musculus ]
Official Symbol : ACO2
Synonyms : ACO2; aconitase 2, mitochondrial; aconitate hydratase, mitochondrial; citrate hydro-lyase; Aco3; Aco-2; D10Wsu183e;
Gene ID : 11429
mRNA Refseq : NM_080633
Protein Refseq : NP_542364
Pathway : Citrate cycle (TCA cycle), organism-specific biosystem; Citrate cycle (TCA cycle), conserved biosystem; Citrate cycle, first carbon oxidation, oxaloacetate =>2-oxoglutarate, organism-specific biosystem; Citrate cycle, first carbon oxidation, oxaloacetate => 2-oxoglutarate, conserved biosystem; Citric acid cycle (TCA cycle), organism-specific biosystem;
Function : 3 iron, 4 sulfur cluster binding; 4 iron, 4 sulfur cluster binding; aconitate hydratase activity; aconitate hydratase activity; citrate hydro-lyase (cis-aconitate-forming) activity; iron ion binding; iron-sulfur cluster binding; isocitrate hydro-lyase (cis-aconitate-forming) activity; lyase activity; metal ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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What is the function of ACO2? 12/29/2022

ACO2 is involved in the TCA cycle, which is a critical process for energy production in cells. Specifically, it catalyzes the conversion of citrate to isocitrate, which is a crucial step in the cycle.

Can ACO2 mutations cause disease? 12/11/2022

Yes, mutations in the ACO2 gene have been associated with several disorders, including infantile cerebellar-retinal degeneration and mitochondrial encephalopathy. These conditions are characterized by a range of symptoms, including neurological and developmental abnormalities.

Can ACO2 be targeted for medical treatment?  10/05/2021

ACO2 is a potential target for cancer treatment, as it is overexpressed in several types of cancer cells. However, the development of drugs that target ACO2 is still in the early stages of research.

How does ACO2 differ from ACO1? 04/28/2021

ACO1 and ACO2 are two isoforms of aconitate hydratase, which catalyze the same reaction in the TCA cycle. However, ACO2 is primarily located in the mitochondria of cells, while ACO1 is located in the cytoplasm.

Is ACO2 involved in iron metabolism? 10/30/2018

Unlike ACO1, ACO2 is not involved in iron metabolism. Its role is primarily in energy production through the TCA cycle.

What happens if ACO2 is deficient? 09/26/2018

ACO2 deficiency has been associated with several disorders, including infantile cerebellar-retinal degeneration and mitochondrial encephalopathy. These conditions are characterized by various neurological and developmental abnormalities.

What is the structure of ACO2? 06/23/2018

ACO2 is a homodimeric enzyme composed of two identical subunits. Each subunit contains a catalytic domain and a regulatory domain, which work together to catalyze the isomerization of citrate to isocitrate.

How is ACO2 measured in the laboratory?  10/02/2017

ACO2 activity can be measured in the laboratory using a variety of techniques, including spectrophotometric assays and enzymatic activity assays. These assays typically involve measuring the rate of citrate isomerization to isocitrate in the presence of ACO2 and other necessary cofactors.

How is the activity of ACO2 regulated? 09/29/2017

ACO2 activity is regulated by a variety of factors, including substrate availability, pH, and post-translational modifications such as phosphorylation. Additionally, ACO2 activity can be modulated by other enzymes and metabolic pathways in the cell.

Does ACO2 have any other functions in addition to energy production? 06/30/2015

While ACO2 is primarily known for its role in energy production, recent research suggests that it may also play a role in other cellular processes. For example, studies have shown that ACO2 may be involved in regulating the immune response and cellular metabolism.

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