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Recombinant Human PAH cell lysate

Cat.No. : PAH-461HCL
Product Overview : Human PAH / PH derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Baculovirus-Insect Cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : PAH phenylalanine hydroxylase [ Homo sapiens ]
Official Symbol : PAH
Synonyms : PAH; phenylalanine hydroxylase; phenylalanine-4-hydroxylase; PH; phenylalanine 4 monooxygenase; phe-4-monooxygenase; phenylalanine 4-monooxygenase; PKU; PKU1;
Gene ID : 5053
mRNA Refseq : NM_000277
Protein Refseq : NP_000268
MIM : 612349
UniProt ID : P00439
Chromosome Location : 12q22-q24.2
Pathway : Biogenic Amine Synthesis, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Phenylalanine and tyrosine catabolism, organism-specific biosystem; Phenylalanine metabolism, organism-specific biosystem; Phenylalanine metabolism, conserved biosystem;
Function : amino acid binding; iron ion binding; metal ion binding; phenylalanine 4-monooxygenase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Can PAH be treated with medications? 05/09/2022

Yes, several medications are available to manage the symptoms and slow the progression of PAH.

How can a better understanding of PAH proteins benefit patients with PAH? 01/17/2022

Understanding the role of proteins in PAH can lead to the development of more targeted and effective treatments, improving the quality of life for patients with PAH.

Are there specific biomarkers related to PAH that involve protein expression? 11/27/2021

Yes, certain biomarkers, such as brain natriuretic peptide (BNP) and endothelin-1, can be indicative of PAH.

Are there any protein-targeted therapies for PAH? 10/25/2020

Research is ongoing in the development of protein-targeted therapies for PAH, but no specific protein-targeted drugs are widely used as of my last update in January 2022.

How can proteins be used in the diagnosis and prognosis of PAH? 05/01/2019

Protein biomarkers can help in early diagnosis, and monitoring their levels can aid in assessing disease progression and response to treatment.

Customer Reviews (3)

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Reviews
06/20/2022

    I highly recommend the use of the PAH protein in various experimental applications.

    03/23/2022

      Considering its outstanding performance across multiple assays, I confidently endorse the inclusion of the PAH protein in diverse research studies.

      12/27/2021

        the PAH protein has been successfully utilized in protein electron microscopy structure analysis, providing valuable insights into molecular structures and interactions.

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