Recombinant Human PAH cell lysate
Cat.No. : | PAH-461HCL |
Product Overview : | Human PAH / PH derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Baculovirus-Insect Cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | PAH phenylalanine hydroxylase [ Homo sapiens ] |
Official Symbol : | PAH |
Synonyms : | PAH; phenylalanine hydroxylase; phenylalanine-4-hydroxylase; PH; phenylalanine 4 monooxygenase; phe-4-monooxygenase; phenylalanine 4-monooxygenase; PKU; PKU1; |
Gene ID : | 5053 |
mRNA Refseq : | NM_000277 |
Protein Refseq : | NP_000268 |
MIM : | 612349 |
UniProt ID : | P00439 |
Chromosome Location : | 12q22-q24.2 |
Pathway : | Biogenic Amine Synthesis, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Phenylalanine and tyrosine catabolism, organism-specific biosystem; Phenylalanine metabolism, organism-specific biosystem; Phenylalanine metabolism, conserved biosystem; |
Function : | amino acid binding; iron ion binding; metal ion binding; phenylalanine 4-monooxygenase activity; |
Products Types
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◆ Assay kits | ||
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionYes, several medications are available to manage the symptoms and slow the progression of PAH.
Understanding the role of proteins in PAH can lead to the development of more targeted and effective treatments, improving the quality of life for patients with PAH.
Yes, certain biomarkers, such as brain natriuretic peptide (BNP) and endothelin-1, can be indicative of PAH.
Research is ongoing in the development of protein-targeted therapies for PAH, but no specific protein-targeted drugs are widely used as of my last update in January 2022.
Protein biomarkers can help in early diagnosis, and monitoring their levels can aid in assessing disease progression and response to treatment.
Customer Reviews (3)
Write a reviewI highly recommend the use of the PAH protein in various experimental applications.
Considering its outstanding performance across multiple assays, I confidently endorse the inclusion of the PAH protein in diverse research studies.
the PAH protein has been successfully utilized in protein electron microscopy structure analysis, providing valuable insights into molecular structures and interactions.
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