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Recombinant Mouse PDPN cell lysate

Cat.No. : PDPN-470MCL
Product Overview : Mouse PDPN / Podoplanin derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Baculovirus-Insect Cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Pdpn podoplanin [ Mus musculus ]
Official Symbol : PDPN
Synonyms : PDPN; podoplanin; aggrus; PA2.26 antigen; glycoprotein 38; transmembrane glycoprotein E11; T1a; Gp38; OTS-8; T1alpha; RANDAM-2; T1-alpha;
Gene ID : 14726
mRNA Refseq : NM_010329
Protein Refseq : NP_034459
Function : NOT amino acid transmembrane transporter activity; NOT folic acid transporter activity; NOT water channel activity; NOT water transmembrane transporter activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What role does PDPN play in cancer progression, particularly in terms of tumor cell migration and metastasis? 07/24/2022

PDPN facilitates cancer progression by promoting tumor cell migration and enhancing metastatic potential.

How does PDPN contribute to the development and maintenance of lymphatic vessels? 05/20/2022

PDPN is crucial for lymphatic vessel development and maintenance, aiding in lymphatic system function.

How does the alteration of PDPN expression impact the pathogenesis of diseases like fibrosis and lymphedema? 03/17/2021

Changes in PDPN expression contribute to fibrosis and lymphedema pathogenesis, affecting tissue structure and fluid balance.

What is the significance of PDPN in the formation and stability of blood-lymphatic vessel junctions? 07/31/2020

PDPN is significant in forming and stabilizing blood-lymphatic vessel junctions, ensuring proper vascular function.

What are the mechanisms by which PDPN modulates platelet aggregation and blood coagulation? 04/14/2020

PDPN affects platelet aggregation and blood coagulation, playing a role in thrombosis and hemostasis.

How does PDPN expression affect the interaction between tumor cells and the immune system? 02/15/2019

PDPN expression on tumor cells alters their interaction with the immune system, impacting immune evasion.

How does PDPN influence wound healing and tissue regeneration processes? 04/10/2018

PDPN plays a role in wound healing and tissue regeneration, influencing cell migration and tissue repair.

Customer Reviews (3)

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Reviews
01/31/2021

    Swift protein expression optimization support; greatly appreciated.

    08/19/2019

      Accurate protein structural characterization; indispensable for our research.

      12/19/2018

        Reliable mass spectrometry analysis; pivotal for proteomics experiments.

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