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Recombinant Human TP63 cell lysate

Cat.No.: TP63-472HCL
Product Overview: Human P63 / TP63 / Tumor derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
Source: Baculovirus-Insect Cells
Species: Human
Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability: Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name: TP63 tumor protein p63 [ Homo sapiens ]
Official Symbol: TP63
Synonyms: TP63; tumor protein p63; TP53CP, TP53L, TP73L, tumor protein p53 competing protein , tumor protein p53 like , tumor protein p73 like; tumor protein 63; EEC3; KET; NBP; OFC8; p51; p53CP; p63; p73H; p73L; SHFM4; CUSP; transformation-related protein 63; tumor protein p53-competing protein; amplified in squamous cell carcinoma; chronic ulcerative stomatitis protein; keratinocyte transcription factor KET; tumor protein p63 deltaN isoform delta; AIS; LMS; RHS; p40; TP53L; TP73L; TP53CP; B(p51A); B(p51B);
Gene ID: 8626
mRNA Refseq: NM_001114978
Protein Refseq: NP_001108450
MIM: 603273
UniProt ID: Q9H3D4
Chromosome Location: 3q27-q29
Pathway: Apoptosis, organism-specific biosystem; Direct p53 effectors, organism-specific biosystem; TP53 network, organism-specific biosystem;
Function: DNA binding; chromatin binding; chromatin binding; damaged DNA binding; double-stranded DNA binding; identical protein binding; metal ion binding; p53 binding; protein binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; transcription regulatory region DNA binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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