Recombinant Human NAMPT cell lysate
Cat.No. : | NAMPT-491HCL |
Product Overview : | Human NAMPT / PBEF derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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- Gene Information
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Source : | Baculovirus-Insect Cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | NAMPT nicotinamide phosphoribosyltransferase [ Homo sapiens ] |
Official Symbol : | NAMPT |
Synonyms : | NAMPT; nicotinamide phosphoribosyltransferase; PBEF1, pre B cell colony enhancing factor 1; PBEF; visfatin; NAmPRTase; pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; pre-B-cell colony-enhancing factor 1; VF; PBEF1; VISFATIN; 1110035O14Rik; MGC117256; DKFZp666B131; |
Gene ID : | 10135 |
mRNA Refseq : | NM_005746 |
Protein Refseq : | NP_005737 |
MIM : | 608764 |
UniProt ID : | P43490 |
Chromosome Location : | 7q22.2 |
Pathway : | Adipogenesis, organism-specific biosystem; BMAL1:CLOCK/NPAS2 Activates Circadian Expression, organism-specific biosystem; Circadian Clock, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of vitamins and cofactors, organism-specific biosystem; Metabolism of water-soluble vitamins and cofactors, organism-specific biosystem; NAD biosynthesis III, organism-specific biosystem; |
Function : | cytokine activity; nicotinamide phosphoribosyltransferase activity; nicotinate phosphoribosyltransferase activity; nicotinate-nucleotide diphosphorylase (carboxylating) activity; transferase activity, transferring glycosyl groups; |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionThe types of mutations in NAMPT include point mutations, insertions/deletions, duplications, etc., which can cause structural and functional abnormalities of the protein.
Some diseases related to NAD+ metabolism can be treated by regulating the level of NAMPT, such as by inhibiting its activity or regulating its expression.
Studying the regulatory mechanism of NAMPT requires a comprehensive use of various experimental methods and techniques, such as gene knockout, transcriptome analysis, and protein-protein interactions.
NAMPT has a complex relationship with other genes or proteins, and can interact with other genes or proteins and participate in a variety of biochemical reactions.
NAMPT plays an important role in cellular energy metabolism by catalyzing the synthesis of NAD+, an important intracellular coenzyme involved in various reactions of energy metabolism.
Mutations in NAMPT can be detected and analyzed by methods such as whole-genome sequencing or target region sequencing to understand the impact of mutations on protein structure and function.
Customer Reviews (3)
Write a reviewDoes not trigger an immune response.
Methods such as ELISA or immunostaining perform well.
Easy to use, high stability, remarkable effect.
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