Recombinant Human FUT8 cell lysate
Cat.No. : | FUT8-497HCL |
Product Overview : | Human FUT8 (aa 68-575) derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Baculovirus-Insect Cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | FUT8 fucosyltransferase 8 (alpha (1,6) fucosyltransferase) [ Homo sapiens ] |
Official Symbol : | FUT8 |
Synonyms : | FUT8; fucosyltransferase 8 (alpha (1,6) fucosyltransferase); alpha-(1,6)-fucosyltransferase; alpha1-6FucT; glycoprotein 6-alpha-L-fucosyltransferase; GDP-fucose--glycoprotein fucosyltransferase; GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase; MGC26465; |
Gene ID : | 2530 |
mRNA Refseq : | NM_004480 |
Protein Refseq : | NP_004471 |
MIM : | 602589 |
UniProt ID : | Q9BYC5 |
Chromosome Location : | 14q24.3 |
Pathway : | Asparagine N-linked glycosylation, organism-specific biosystem; Glycosaminoglycan biosynthesis - keratan sulfate, organism-specific biosystem; Glycosaminoglycan biosynthesis - keratan sulfate, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; N-Glycan biosynthesis, organism-specific biosystem; N-Glycan biosynthesis, conserved biosystem; |
Function : | SH3 domain binding; glycoprotein 6-alpha-L-fucosyltransferase activity; transferase activity, transferring glycosyl groups; |
Products Types
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FUT8-31H | Active Recombinant Human FUT8 Protein (AA 41-575), N-6×His/GFP tagged | +Inquiry |
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FUT8-1590R | Recombinant Rhesus Macaque FUT8 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
FUT8-001HCL | Recombinant Hamster FUT8 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionYes, altering FUT8 activity can impact cancer cell growth and metastasis, making it a potential target for cancer therapies.
One challenge is the specificity of FUT8 inhibitors, ensuring they do not affect other essential glycosylation processes in the body.
Yes, abnormal glycosylation patterns due to FUT8 alterations can serve as biomarkers for diseases like cancer, aiding in early diagnosis.
FUT8-mediated glycosylation of antibodies can enhance their stability and effector functions, making them more effective in treating diseases like cancer and autoimmune disorders.
Yes, deficiencies in FUT8 have been associated with certain types of cancers and developmental disorders.
Customer Reviews (3)
Write a reviewWith the manufacturer's outstanding technical support, you can be assured that any challenges you encounter will be efficiently addressed.
Researchers who have utilized the FUT8 protein laud its reliability and effectiveness in their studies.
Its superior quality, combined with the manufacturer's exceptional technical support, provides researchers with the confidence they need to conduct successful experiments and obtain meaningful results.
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