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Recombinant Human EED cell lysate

Cat.No. : EED-531HCL
Product Overview : Human EED / Embryonic Ectoderm Development derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Baculovirus-Insect Cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : EED embryonic ectoderm development [ Homo sapiens ]
Official Symbol : EED
Synonyms : EED; embryonic ectoderm development; polycomb protein EED; HEED; WAIT 1; WD protein associating with integrin cytoplasmic tails 1; WAIT1;
Gene ID : 8726
mRNA Refseq : NM_003797
Protein Refseq : NP_003788
MIM : 605984
UniProt ID : O75530
Chromosome Location : 11q14.2-q22.3
Function : chromatin binding; histone methyltransferase activity; identical protein binding; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What is the impact of EED dysregulation on the development of cancer, especially in terms of epigenetic alterations? 08/18/2022

Dysregulation of EED can lead to cancer, largely through epigenetic alterations and disrupted gene regulation.

How does EED contribute to the maintenance of pluripotency and self-renewal in stem cells? 04/26/2022

EED helps maintain stem cell pluripotency and self-renewal capabilities, crucial for embryonic development.

How does EED interact with the Polycomb repressive complex 2 (PRC2) in chromatin remodeling? 05/05/2021

EED works with PRC2 in chromatin remodeling, influencing gene silencing and cellular identity.

How do alterations in EED expression or function affect developmental disorders and congenital anomalies? 10/26/2020

Changes in EED function impact developmental processes, potentially leading to congenital anomalies and disorders.

How does EED regulate epigenetic modifications, particularly histone methylation, in gene expression? 10/18/2020

EED regulates gene expression by modulating histone methylation, affecting epigenetic landscapes.

What role does EED play in the development and differentiation of embryonic stem cells? 06/28/2020

EED is essential in embryonic stem cell development, guiding differentiation processes.

What are the mechanisms by which EED influences cellular aging and senescence? 01/25/2019

EED affects cellular aging and senescence, playing a role in age-related epigenetic changes.

Customer Reviews (3)

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Reviews
12/25/2021

    Precise protein quantification, ensures data accuracy in our experiments.

    07/03/2020

      Dependable peptide synthesis, accelerates our projects effectively.

      03/02/2020

        Timely antibody validation, a valuable asset for our lab.

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