Recombinant Human EED cell lysate
Cat.No. : | EED-531HCL |
Product Overview : | Human EED / Embryonic Ectoderm Development derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Baculovirus-Insect Cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | EED embryonic ectoderm development [ Homo sapiens ] |
Official Symbol : | EED |
Synonyms : | EED; embryonic ectoderm development; polycomb protein EED; HEED; WAIT 1; WD protein associating with integrin cytoplasmic tails 1; WAIT1; |
Gene ID : | 8726 |
mRNA Refseq : | NM_003797 |
Protein Refseq : | NP_003788 |
MIM : | 605984 |
UniProt ID : | O75530 |
Chromosome Location : | 11q14.2-q22.3 |
Function : | chromatin binding; histone methyltransferase activity; identical protein binding; protein binding; |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionDysregulation of EED can lead to cancer, largely through epigenetic alterations and disrupted gene regulation.
EED helps maintain stem cell pluripotency and self-renewal capabilities, crucial for embryonic development.
EED works with PRC2 in chromatin remodeling, influencing gene silencing and cellular identity.
Changes in EED function impact developmental processes, potentially leading to congenital anomalies and disorders.
EED regulates gene expression by modulating histone methylation, affecting epigenetic landscapes.
EED is essential in embryonic stem cell development, guiding differentiation processes.
EED affects cellular aging and senescence, playing a role in age-related epigenetic changes.
Customer Reviews (3)
Write a reviewPrecise protein quantification, ensures data accuracy in our experiments.
Dependable peptide synthesis, accelerates our projects effectively.
Timely antibody validation, a valuable asset for our lab.
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