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Recombinant Human PTPN2 cell lysate

Cat.No. : PTPN2-567HCL
Product Overview : Human PTPN2 / PTPT / TC-PTP (aa 1-314) derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Baculovirus-Insect Cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : PTPN2 protein tyrosine phosphatase, non-receptor type 2 [ Homo sapiens ]
Official Symbol : PTPN2
Synonyms : PTPN2; protein tyrosine phosphatase, non-receptor type 2; PTPT; tyrosine-protein phosphatase non-receptor type 2; TC PTP; TCELLPTP; TCPTP; T-cell protein tyrosine phosphatase; PTN2; TC-PTP;
Gene ID : 5771
mRNA Refseq : NM_001207013
Protein Refseq : NP_001193942
MIM : 176887
UniProt ID : P17706
Chromosome Location : 18p11.3-p11.2
Pathway : Cytokine Signaling in Immune system, organism-specific biosystem; Immune System, organism-specific biosystem; Interferon Signaling, organism-specific biosystem; Interferon gamma signaling, organism-specific biosystem; Regulation of IFNG signaling, organism-specific biosystem;
Function : hydrolase activity; non-membrane spanning protein tyrosine phosphatase activity; protein binding; protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What is the therapeutic effect of specific PTPN2 inhibitors on inflammatory diseases? 09/30/2022

PTPN2 inhibitors can inhibit certain signaling pathways in the inflammatory response, thereby playing a role in the treatment of inflammatory diseases.

Is there a soluble PTPN2 protein? 01/06/2022

Yes, soluble PTPN2 proteins exist that can be detected in extracellular fluids and may play a role in regulating cell-to-cell communication and signal transduction.

What is the modulating effect of soluble PTPN2 protein on immune response? 03/17/2021

PTPN2 protein can interact with receptors on the surface of immune cells to regulate the activation and function of immune cells, thereby playing a role in regulating immune responses.

What is the role of PTPN2 protein in autoimmune diseases? 01/15/2021

Studies have shown that PTPN2 protein plays a certain role in autoimmune diseases, and its expression level may be related to the occurrence and progression of the disease.

Are specific PTPN2 inhibitors useful for the treatment of autoimmune diseases? 11/02/2020

Specific PTPN2 inhibitors can inhibit certain abnormal signaling pathways in autoimmune diseases, thereby playing a role in the treatment of autoimmune diseases.

How does PTPN2 protein regulate immune response? 04/16/2020

This protein can interact with a variety of immune molecules, such as T cell receptors (TCRs) and B cell receptors (BCRs), to regulate immune responses by regulating their phosphorylation status.

Customer Reviews (3)

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Reviews
10/06/2022

    Repeatability is good, and the results of multiple experiments are consistent.

    08/10/2022

      The impurities are very well removed and the purity is very high.

      04/14/2021

        Performance is very stable and the price is very reasonable. I would definitely buy this product again.

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