Recombinant Human KITLG cell lysate
Cat.No. : | KITLG-571HCL |
Product Overview : | Human KITLG / Kit ligand / Steel factor (aa 1-189 ) derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Baculovirus-Insect Cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | KITLG KIT ligand [ Homo sapiens ] |
Official Symbol : | KITLG |
Synonyms : | KITLG; KIT ligand; MGF; kit ligand; familial progressive hyperpigmentation 2; FPH2; Kitl; KL 1; mast cell growth factor; SCF; SF; steel factor; stem cell factor; c-Kit ligand; KL-1; SHEP7; kit-ligand; DKFZp686F2250; |
Gene ID : | 4254 |
mRNA Refseq : | NM_000899 |
Protein Refseq : | NP_000890 |
MIM : | 184745 |
UniProt ID : | P21583 |
Chromosome Location : | 12q22 |
Pathway : | C-MYB transcription factor network, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Kit Receptor Signaling Pathway, organism-specific biosystem; Melanogenesis, organism-specific biosystem; |
Function : | cytokine activity; growth factor activity; protein binding; stem cell factor receptor binding; |
Products Types
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KITLG-2737D | Recombinant Dog KITLG Protein, His-tagged | +Inquiry |
KITLG-4356R | Recombinant Rabbit KITLG Protein | +Inquiry |
KITLG-39C | Recombinant Chicken KITLG Protein | +Inquiry |
◆ Lysates | ||
KITLG-583MCL | Recombinant Mouse KITLG cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionOverexpression or mutations in KITLG and its receptor have been implicated in various cancers, including gastrointestinal stromal tumors (GISTs), mastocytosis, and certain leukemias.
Given its role in cell proliferation and differentiation, KITLG has been explored as a therapeutic agent in stem cell transplantation settings, and its pathway is a target in certain cancer treatments.
Dysregulation or mutations in KITLG have been associated with certain disorders like piebaldism, a genetic condition characterized by unpigmented patches of skin and hair.
Upon binding to c-KIT, KITLG activates the SCF signaling pathways, which include the MAPK, PI3K, and JAK/STAT pathways, influencing cell survival, proliferation, and differentiation.
KITLG binds to the c-KIT receptor, a tyrosine kinase receptor, leading to its dimerization and autophosphorylation, which activates downstream signaling pathways.
Given its role in cell proliferation and differentiation, KITLG has been explored as a therapeutic agent in stem cell transplantation settings, and its pathway is a target in certain cancer treatments.
KITLG and its receptor c-KIT are involved in melanogenesis. Their interaction regulates the proliferation and migration of melanocytes, influencing skin, hair, and eye pigmentation.
Customer Reviews (3)
Write a reviewReliable for immunoprecipitation assay.
No contamination, very clean.
Worked perfectly in Western.
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