Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Mouse KITLG cell lysate

Cat.No. : KITLG-583MCL
Product Overview : Mouse KITL / Kitlg / Stem cell factor derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
  • Specification
  • Gene Information
  • Related Products
Source : Baculovirus-Insect Cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Kitl kit ligand [ Mus musculus ]
Official Symbol : Kitl
Synonyms : Gb; SF; Sl; Clo; Con; Mgf; SCF; SLF; blz; Kitlg; contrasted; kit ligand; cloud gray; C-kit ligand; Steel factor; grizzle-belly; stem; cell factor; mast cell growth factor; hematopoietic growth factor KL
Gene ID : 17311
mRNA Refseq : NM_013598
Protein Refseq : NP_038626
UniProt ID : P20826
Chromosome Location : 10 D1; 10 51.4 Cm
Pathway : Adaptive Immune System, organism-specific biosystem; Downstream signaling events of B Cell Receptor (BCR), organism-specific biosystem; Immune System, organism-specific biosystem
Function : cytokine activity; growth factor activity; stem cell factor receptor binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (7)

Ask a question
How does KITLG influence stem cell factor (SCF) signaling pathways? 11/10/2022

Overexpression or mutations in KITLG and its receptor have been implicated in various cancers, including gastrointestinal stromal tumors (GISTs), mastocytosis, and certain leukemias.

What are the implications of KITLG in oncogenesis and tumor progression? 07/20/2022

Given its role in cell proliferation and differentiation, KITLG has been explored as a therapeutic agent in stem cell transplantation settings, and its pathway is a target in certain cancer treatments.

What role does KITLG play in melanogenesis and pigmentation? 01/17/2022

Dysregulation or mutations in KITLG have been associated with certain disorders like piebaldism, a genetic condition characterized by unpigmented patches of skin and hair.

Are there any known disorders associated with mutations or dysregulation of KITLG? 06/22/2020

Upon binding to c-KIT, KITLG activates the SCF signaling pathways, which include the MAPK, PI3K, and JAK/STAT pathways, influencing cell survival, proliferation, and differentiation.

What is the primary biological function of KITLG in the hematopoietic system? 04/12/2020

KITLG binds to the c-KIT receptor, a tyrosine kinase receptor, leading to its dimerization and autophosphorylation, which activates downstream signaling pathways.

How has KITLG been explored as a therapeutic target or agent in clinical settings? 02/10/2020

Given its role in cell proliferation and differentiation, KITLG has been explored as a therapeutic agent in stem cell transplantation settings, and its pathway is a target in certain cancer treatments.

How does KITLG interact with its receptor, c-KIT? 12/21/2019

KITLG and its receptor c-KIT are involved in melanogenesis. Their interaction regulates the proliferation and migration of melanocytes, influencing skin, hair, and eye pigmentation.

Customer Reviews (3)

Write a review
Reviews
01/23/2022

    Reliable for immunoprecipitation assay.

    03/01/2021

      No contamination, very clean.

      02/09/2020

        Worked perfectly in Western.

        Ask a Question for All Kitlg Products

        Required fields are marked with *

        My Review for All Kitlg Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends