Recombinant Human PDK1 cell lysate
Cat.No. : | PDK1-603HCL |
Product Overview : | Human PDK-1 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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Source : | Baculovirus-Insect Cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | PDK1 pyruvate dehydrogenase kinase, isozyme 1 [ Homo sapiens ] |
Official Symbol : | PDK1 |
Synonyms : | PDK1; pyruvate dehydrogenase kinase, isozyme 1; pyruvate dehydrogenase kinase, isoenzyme 1; mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1; |
Gene ID : | 5163 |
mRNA Refseq : | NM_002610 |
Protein Refseq : | NP_002601 |
MIM : | 602524 |
UniProt ID : | Q15118 |
Chromosome Location : | 2q31.1 |
Pathway : | EPO Receptor Signaling, organism-specific biosystem; ErbB signaling pathway, organism-specific biosystem; Fc epsilon RI signaling pathway, organism-specific biosystem; Fc epsilon RI signaling pathway, conserved biosystem; Hepatitis C, organism-specific biosystem; Hepatitis C, conserved biosystem; Metabolism, organism-specific biosystem; |
Function : | ATP binding; nucleotide binding; protein complex binding; protein heterodimerization activity; protein homodimerization activity; protein kinase activity; pyruvate dehydrogenase (acetyl-transferring) kinase activity; transferase activity; two-component sensor activity; |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionMay play a role in the occurrence of obesity, and the current research conclusions still need to be further verified.
PDK1 is thought to be associated with the occurrence and progression of cardiovascular disease, and may affect the health of the cardiovascular system by regulating the glucose metabolism of cardiomyocytes and the activity of phosphatase. However, further research and validation are needed.
Participates in a variety of signaling pathways, such as Akt and PKC, and affects the transmission and regulation of various signaling pathways by regulating the kinase activities of related receptors and enzymes.
PDK1 has abnormal expression in a variety of tumors, such as breast cancer, pancreatic cancer, colorectal cancer, etc., and is one of the important markers of tumor occurrence and prognosis.
The plays an important role in regulating cell metabolism and plays an important role in energy metabolism by regulating the phosphorylation state of various enzymes.
It has been identified as a potential therapeutic target for new diabetes, possibly by improving insulin signaling and glycogen synthesis, but further exploration is needed.
Customer Reviews (3)
Write a reviewPDK1 production process is strictly controlled and the quality of the product is guaranteed.
Reproducibility of PDK1 is simply unrivaled, with identical results in every experiment or application, demonstrating its high stability and reliability.
The catalytic activity of PDK1 is excellent, which can simplify complex chemical reactions and improve yields, and its scientific research value is self-evident.
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