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Recombinant Human TRIB3 cell lysate

Cat.No. : TRIB3-657HCL
Product Overview : Human TRIB3 / NIPK derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Baculovirus-Insect Cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : TRIB3 tribbles homolog 3 (Drosophila) [ Homo sapiens ]
Official Symbol : TRIB3
Synonyms : TRIB3; tribbles homolog 3 (Drosophila); C20orf97, chromosome 20 open reading frame 97; tribbles homolog 3; dJ1103G7.3; TRB3; TRB-3; p65-interacting inhibitor of NF-kappaB; p65-interacting inhibitor of NF-kappa-B; neuronal cell death inducible putative kinase; neuronal cell death-inducible putative kinase; NIPK; SINK; SKIP3; C20orf97;
Gene ID : 57761
mRNA Refseq : NM_021158
Protein Refseq : NP_066981
MIM : 607898
UniProt ID : Q96RU7
Chromosome Location : 20p13-p12.2
Pathway : Activation of PKB, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Adipogenesis, organism-specific biosystem; CD28 co-stimulation, organism-specific biosystem; CD28 dependent PI3K/Akt signaling, organism-specific biosystem; Costimulation by the CD28 family, organism-specific biosystem; Disease, organism-specific biosystem;
Function : ATP binding; enzyme binding; NOT kinase activity; protein binding; protein kinase binding; protein kinase binding; protein kinase inhibitor activity; transcription corepressor activity; ubiquitin protein ligase binding; ubiquitin-protein ligase regulator activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How does TRIB3 contribute to cancer progression? 05/29/2021

TRIB3 has been implicated in promoting cancer cell proliferation, survival, and metastasis in various types of cancer.

Can TRIB3 be a potential therapeutic target for cardiovascular diseases? 01/15/2020

Targeting TRIB3 may have implications for the treatment of cardiovascular diseases, including atherosclerosis.

How does TRIB3 affect diabetes? 04/24/2018

TRIB3 has been shown to regulate insulin signaling and glucose metabolism, and its overexpression has been linked to insulin resistance and type 2 diabetes.

Can targeting TRIB3 be a potential strategy for cancer therapy? 02/18/2018

Yes, targeting TRIB3 may be a potential strategy for inhibiting cancer cell growth and metastasis.

What are the clinical implications of TRIB3 protein? 04/23/2017

TRIB3 has been implicated in several diseases, including diabetes, cancer, and cardiovascular diseases.

Customer Reviews (3)

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Reviews
02/16/2021

    The manufacturer also values customer satisfaction and strives to maintain a strong relationship with researchers.

    07/29/2019

      Whether it's troubleshooting experimental issues, providing guidance on experimental design, or answering any inquiries related to the TRIB3 protein, the manufacturer is committed to helping researchers overcome challenges and achieve their scientific goals.

      08/05/2016

        They actively seek feedback and incorporate it into their product development process, continuously improving the quality and performance of the TRIB3 protein.

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