Recombinant Human TGM2 cell lysate
Cat.No. : | TGM2-686HCL |
Product Overview : | Human TGM2 / TGC / Transglutaminase-2 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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- Gene Information
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Source : | Baculovirus-Insect Cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | TGM2 transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase) [ Homo sapiens ] |
Official Symbol : | TGM2 |
Synonyms : | TGM2; transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase); protein-glutamine gamma-glutamyltransferase 2; TGC; TG(C); TGase C; TGase H; TGase-2; TGase-H; C polypeptide; transglutaminase C; transglutaminase H; transglutaminase-2; tissue transglutaminase; protein-glutamine-gamma-glutamyltransferase; TG2; GNAH; G-ALPHA-h; |
Gene ID : | 7052 |
mRNA Refseq : | NM_004613 |
Protein Refseq : | NP_004604 |
MIM : | 190196 |
UniProt ID : | P21980 |
Chromosome Location : | 20q12 |
Pathway : | Huntingtons disease, organism-specific biosystem; Huntingtons disease, conserved biosystem; Thromboxane A2 receptor signaling, organism-specific biosystem; |
Function : | GTP binding; metal ion binding; protein binding; protein domain specific binding; protein-glutamine gamma-glutamyltransferase activity; transferase activity, transferring acyl groups; |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionTGM2 levels and activity can serve as diagnostic markers in certain conditions, providing insights into the progression and severity of diseases.
TGM2 has been associated with cardiovascular diseases, contributing to the development of atherosclerosis and vascular calcification.
TGM2 plays a role in fibrosis by promoting the cross-linking of extracellular matrix proteins, contributing to tissue stiffness and impaired organ function.
TGM2 is involved in modulating inflammation and immune responses, influencing processes such as cytokine production and leukocyte adhesion.
Genetic variations in TGM2 have been identified, and certain mutations may influence susceptibility to specific diseases or alter the enzyme's functionality.
Customer Reviews (3)
Write a reviewThe TGM2 protein has demonstrated its efficacy in protein electron microscopy structure analysis.
Whether utilized in protein-protein interaction studies or enzymatic assays, this protein exhibits robust performance, enabling accurate data generation and analysis.
This cutting-edge technique allows for the visualization and characterization of protein structures at the atomic level, providing insights into their function and potential therapeutic targets.
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