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Recombinant Human TGM2 cell lysate

Cat.No. : TGM2-686HCL
Product Overview : Human TGM2 / TGC / Transglutaminase-2 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Baculovirus-Insect Cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : TGM2 transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase) [ Homo sapiens ]
Official Symbol : TGM2
Synonyms : TGM2; transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase); protein-glutamine gamma-glutamyltransferase 2; TGC; TG(C); TGase C; TGase H; TGase-2; TGase-H; C polypeptide; transglutaminase C; transglutaminase H; transglutaminase-2; tissue transglutaminase; protein-glutamine-gamma-glutamyltransferase; TG2; GNAH; G-ALPHA-h;
Gene ID : 7052
mRNA Refseq : NM_004613
Protein Refseq : NP_004604
MIM : 190196
UniProt ID : P21980
Chromosome Location : 20q12
Pathway : Huntingtons disease, organism-specific biosystem; Huntingtons disease, conserved biosystem; Thromboxane A2 receptor signaling, organism-specific biosystem;
Function : GTP binding; metal ion binding; protein binding; protein domain specific binding; protein-glutamine gamma-glutamyltransferase activity; transferase activity, transferring acyl groups;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Are there diagnostic applications of TGM2 in clinical settings? 02/28/2021

TGM2 levels and activity can serve as diagnostic markers in certain conditions, providing insights into the progression and severity of diseases.

How does TGM2 influence cardiovascular health? 01/26/2021

TGM2 has been associated with cardiovascular diseases, contributing to the development of atherosclerosis and vascular calcification.

How does TGM2 contribute to fibrosis in organ tissues? 10/25/2020

TGM2 plays a role in fibrosis by promoting the cross-linking of extracellular matrix proteins, contributing to tissue stiffness and impaired organ function.

How does TGM2 affect inflammation and immune responses? 09/17/2020

TGM2 is involved in modulating inflammation and immune responses, influencing processes such as cytokine production and leukocyte adhesion.

Are there genetic mutations related to TGM2 that impact clinical outcomes? 07/08/2017

Genetic variations in TGM2 have been identified, and certain mutations may influence susceptibility to specific diseases or alter the enzyme's functionality.

Customer Reviews (3)

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Reviews
12/21/2020

    The TGM2 protein has demonstrated its efficacy in protein electron microscopy structure analysis.

    03/30/2020

      Whether utilized in protein-protein interaction studies or enzymatic assays, this protein exhibits robust performance, enabling accurate data generation and analysis.

      07/19/2018

        This cutting-edge technique allows for the visualization and characterization of protein structures at the atomic level, providing insights into their function and potential therapeutic targets.

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