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Recombinant Human ANGPTL5 cell lysate

Cat.No. : ANGPTL5-704HCL
Product Overview : Human ANGPTL5 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Baculovirus-Insect Cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : ANGPTL5 angiopoietin-like 5 [ Homo sapiens ]
Official Symbol : ANGPTL5
Synonyms : ANGPTL5; angiopoietin-like 5; angiopoietin-related protein 5; angiopoietin-like protein 5;
Gene ID : 253935
mRNA Refseq : NM_178127
Protein Refseq : NP_835228
MIM : 607666
UniProt ID : Q86XS5
Chromosome Location : 11q22.2
Function : receptor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (15)

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Is ANGPTL5 being targeted for drug development? 01/15/2023

While ANGPTL5 shows promise as a therapeutic target, it is still in the early stages of drug development. Researchers are exploring various strategies to target ANGPTL5, such as the development of small molecule inhibitors or antibodies to block its activity. However, it may take several years of research and clinical trials before any ANGPTL5-targeted drugs become available for use.

Is ANGPTL5 expressed in specific tissues? 10/22/2022

ANGPTL5 is expressed in various tissues, but its expression levels can vary. It is primarily expressed in adipose tissue, as well as in the skeletal muscle, liver, and heart. Expression of ANGPTL5 can be influenced by factors such as nutritional status and hormonal signaling, which can affect its biological functions in different tissues.

What are the potential roles of ANGPTL5 in cancer? 12/23/2021

ANGPTL5 has been implicated in cancer development and progression. It has been shown to promote tumor angiogenesis, which is the formation of blood vessels to support tumor growth. ANGPTL5 also appears to play a role in tumor invasion and metastasis. However, the precise mechanisms by which ANGPTL5 exerts its effects in cancer are still being investigated.

Can ANGPTL5 be detected in the bloodstream? 12/19/2020

Yes, ANGPTL5 can be detected in the bloodstream. It has been found to circulate in the plasma, albeit at relatively low levels compared to other ANGPTL family members. The circulating levels of ANGPTL5 can vary depending on factors such as nutritional status and certain diseases.

Is ANGPTL5 associated with any diseases? 11/08/2020

ANGPTL5 has been implicated in various diseases or conditions related to lipid metabolism and angiogenesis. Studies have suggested associations between ANGPTL5 genetic variants and triglyceride levels, obesity, insulin resistance, and cardiovascular disease. However, more research is needed to fully understand the implications of ANGPTL5 in these diseases.

Are there any known interactions of ANGPTL5 with other proteins or molecules? 07/02/2020

ANGPTL5 has been shown to interact with several proteins and molecules. For example, it can interact with integrins, which are cell adhesion molecules involved in angiogenesis. ANGPTL5 has also been found to interact with lipoprotein lipase (LPL), an enzyme involved in lipid metabolism. These interactions contribute to the functions of ANGPTL5 in angiogenesis and lipid regulation.

Are there any known mutations or polymorphisms in the ANGPTL5 gene? 01/31/2020

Yes, several genetic variations in the ANGPTL5 gene have been identified. Some of these variations are associated with altered levels of triglycerides and other lipid parameters in the blood. However, the specific functional consequences of these variants are still being investigated.

Are there any other members of the ANGPTL family? 01/07/2020

Yes, the ANGPTL family consists of several members, including ANGPTL1-8. Each member of the family has distinct functions and expression patterns. For example, ANGPTL1 has been shown to regulate angiogenesis, while ANGPTL3 and ANGPTL4 are involved in lipid metabolism. ANGPTL6 and ANGPTL8 are also associated with metabolic regulation.

What are the known functions of ANGPTL5? 07/11/2019

ANGPTL5 is involved in several biological processes. It has been shown to promote angiogenesis, which is important for tissue remodeling and wound healing. ANGPTL5 also influences adipose tissue metabolism, including the regulation of lipid storage and energy expenditure. Additionally, ANGPTL5 has been implicated in the regulation of glucose metabolism and insulin sensitivity.

Are there any known interactions or relationships between ANGPTL5 and other proteins or molecules? 05/02/2019

ANGPTL5 has been shown to interact with other proteins involved in lipid metabolism and angiogenesis. For example, it has been found to interact with lipoprotein lipase (LPL) and endothelial lipase (EL), both of which are enzymes that play roles in lipid metabolism. These interactions suggest that ANGPTL5 may regulate lipid metabolism through its interactions with these proteins.

Are there any genetic mutations or polymorphisms associated with ANGPTL5? 12/19/2018

There have been studies investigating genetic variations in the ANGPTL5 gene and their potential association with certain diseases or phenotypes. For example, a specific genetic variant in ANGPTL5 has been associated with changes in body fat distribution. However, more research is needed to fully understand the impact of genetic variations in ANGPTL5 and their functional consequences.

Can ANGPTL5 be targeted for therapeutic purposes? 12/15/2018

ANGPTL5 is a potential target for therapeutic intervention in certain diseases. For example, targeting ANGPTL5 in cancer may help inhibit angiogenesis and tumor growth. Additionally, modulating ANGPTL5 levels or activity could potentially be beneficial in metabolic disorders such as obesity and type 2 diabetes. However, more research is needed to fully understand the therapeutic potential and safety of targeting ANGPTL5.

Are there any diseases or disorders associated with ANGPTL5? 10/20/2018

ANGPTL5 has been associated with various diseases and disorders. In addition to its potential role in cancer progression, ANGPTL5 has been implicated in obesity and metabolic disorders. There are some studies suggesting that ANGPTL5 may contribute to the development of insulin resistance and type 2 diabetes. However, further research is needed to fully understand the involvement of ANGPTL5 in these conditions.

Are there any known animal models lacking ANGPTL5? 07/13/2018

Yes, there are animal models, such as mouse models, in which the ANGPTL5 gene has been knocked out or genetically manipulated to decrease its expression. These animal models can be useful for studying the physiological roles of ANGPTL5 and its potential implications in various diseases.

Can ANGPTL5 be a potential therapeutic target? 05/30/2017

ANGPTL5 is being studied as a potential therapeutic target for lipid-related disorders, including hypertriglyceridemia and cardiovascular disease. Researchers are exploring various approaches, such as inhibiting ANGPTL5 activity or modulating its expression, to potentially regulate lipid metabolism and angiogenesis. However, it is important to note that this research is still in its early stages, and further studies are needed to determine the therapeutic potential of targeting ANGPTL5.

Customer Reviews (5)

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Reviews
09/28/2022

    An outstanding aspect of the manufacturer of the ANGPTL5 protein is their commitment to providing excellent technical support.

    03/25/2022

      This high-quality protein serves as an invaluable tool in unraveling the intricate mechanisms governed by ANGPTL5, enabling me to gain deeper insights into its functions and implications.

      12/03/2018

        With their assistance, I can confidently delve into the intricacies of ANGPTL5 and make significant contributions to the field of ANGPTL5-related research.

        11/20/2016

          They consistently stay at the forefront of scientific advancements and customer feedback, allowing them to refine the ANGPTL5 protein based on emerging research trends.

          03/22/2016

            The ANGPTL5 protein exhibits exceptional quality, making it an ideal choice to meet my experimental requirements.

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