Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Rat IL22RA2 cell lysate

Cat.No. : IL22RA2-739RCL
Product Overview : Rat IL22BP / L22RA2 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
  • Specification
  • Gene Information
  • Related Products
Source : Human cells
Species : Rat
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Il22ra2 interleukin 22 receptor, alpha 2 [ Rattus norvegicus ]
Official Symbol : IL22RA2
Synonyms : IL22RA2; interleukin 22 receptor, alpha 2; interleukin-22 receptor subunit alpha-2; IL22BP; IL-22BP; IL-22RA2; IL-22R-alpha-2; IL-22 receptor subunit alpha-2; interleukin-22-binding protein; cytokine receptor family II soluble 1; cytokine receptor family type 2, soluble 1; Crf2-s1;
Gene ID : 444986
mRNA Refseq : NM_001003404
Protein Refseq : NP_001003404
Pathway : Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem;
Function : interleukin-22 binding; interleukin-22 binding; interleukin-22 receptor activity; receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (10)

Ask a question
How does IL22RA2 interact with IL22 at the molecular level? 09/30/2022

IL22RA2 binds to IL22, acting as a decoy receptor.

Can IL22RA2 be targeted for autoimmune disease therapy? 08/23/2022

IL22RA2 is a potential therapeutic target for autoimmune diseases.

What cellular pathways are influenced by IL22RA2? 07/07/2022

IL22RA2 influences the JAK-STAT and NF-κB pathways.

Does IL22RA2 expression correlate with disease severity? 03/16/2022

IL22RA2 expression correlates with disease severity in psoriasis.

What are the kinetics of IL22RA2 and IL22 binding? 01/18/2022

IL22RA2 has high binding kinetics with IL22.

Is IL22RA2 involved in epithelial barrier function? 01/09/2022

IL22RA2 is involved in maintaining epithelial barrier integrity.

How does IL22RA2 expression change during infection? 04/14/2020

IL22RA2 expression is upregulated during bacterial infection.

What is the turnover rate of IL22RA2 in cells? 12/31/2018

The turnover rate of IL22RA2 in cells is approximately 24 hours.

Does IL22RA2 have any alternative splicing variants? 06/13/2018

IL22RA2 has multiple alternative splicing variants.

What post-translational modifications occur on IL22RA2? 03/29/2017

IL22RA2 undergoes phosphorylation.

Customer Reviews (3)

Write a review
Reviews
08/26/2020

    Potent in Th17 activation.

    09/26/2017

      High specificity.

      06/21/2016

        Good for cytokine assays.

        Ask a Question for All IL22RA2 Products

        Required fields are marked with *

        My Review for All IL22RA2 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends