Recombinant Rat IL22RA2 cell lysate
Cat.No. : | IL22RA2-739RCL |
Product Overview : | Rat IL22BP / L22RA2 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Rat |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | Il22ra2 interleukin 22 receptor, alpha 2 [ Rattus norvegicus ] |
Official Symbol : | IL22RA2 |
Synonyms : | IL22RA2; interleukin 22 receptor, alpha 2; interleukin-22 receptor subunit alpha-2; IL22BP; IL-22BP; IL-22RA2; IL-22R-alpha-2; IL-22 receptor subunit alpha-2; interleukin-22-binding protein; cytokine receptor family II soluble 1; cytokine receptor family type 2, soluble 1; Crf2-s1; |
Gene ID : | 444986 |
mRNA Refseq : | NM_001003404 |
Protein Refseq : | NP_001003404 |
Pathway : | Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem; |
Function : | interleukin-22 binding; interleukin-22 binding; interleukin-22 receptor activity; receptor activity; |
Products Types
◆ Recombinant Protein | ||
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Il22ra2-70M | Recombinant Mouse Il22ra2 Protein, His (Fc)-Avi-tagged | +Inquiry |
IL22RA2-100H | Recombinant Human IL22RA2 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
IL22RA2-2586HCL | Recombinant Human IL22RA2 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionIL22RA2 binds to IL22, acting as a decoy receptor.
IL22RA2 is a potential therapeutic target for autoimmune diseases.
IL22RA2 influences the JAK-STAT and NF-κB pathways.
IL22RA2 expression correlates with disease severity in psoriasis.
IL22RA2 has high binding kinetics with IL22.
IL22RA2 is involved in maintaining epithelial barrier integrity.
IL22RA2 expression is upregulated during bacterial infection.
The turnover rate of IL22RA2 in cells is approximately 24 hours.
IL22RA2 has multiple alternative splicing variants.
IL22RA2 undergoes phosphorylation.
Customer Reviews (3)
Write a reviewPotent in Th17 activation.
High specificity.
Good for cytokine assays.
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