Recombinant Human AFM cell lysate
Cat.No. : | AFM-794HCL |
Product Overview : | Human AFM / Afamin derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | AFM afamin [ Homo sapiens ] |
Official Symbol : | AFM |
Synonyms : | AFM; afamin; ALB2; ALBA; alpha-Alb; alpha-albumin; ALF; MGC125338; MGC125339; |
Gene ID : | 173 |
mRNA Refseq : | NM_001133 |
Protein Refseq : | NP_001124 |
MIM : | 104145 |
UniProt ID : | P43652 |
Chromosome Location : | 4q13.3 |
Function : | vitamin E binding; |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionAFM protein plays a role in cellular processes such as cell adhesion, migration, or signaling pathways, and the underlying molecular mechanisms can be investigated using techniques like siRNA knockdown, gene overexpression, or functional assays.
Post-translational modifications or regulatory mechanisms may modulate the activity or function of AFM protein, and these can be studied through site-directed mutagenesis, phosphoproteomics analysis, or biochemical assays.
Downstream signaling pathways or cellular responses triggered by AFM protein activation can be studied through techniques such as Western blotting, gene expression analysis, or functional assays specific to the signaling pathways of interest.
The cellular localization of AFM protein can be experimentally determined using techniques such as immunofluorescence microscopy or subcellular fractionation.
The role of AFM protein in tissue development, organogenesis, or disease progression can be explored using model systems such as animal models, organoid cultures, or tissue engineering approaches.
The binding partners or interacting molecules of AFM protein can be identified through approaches like co-immunoprecipitation, protein-protein interaction assays, or mass spectrometry.
Modulating AFM protein activity or expression may have implications for cell behavior or tissue regeneration, and the therapeutic potential can be assessed using in vitro or in vivo models combined with functional assays or tissue engineering approaches.
Genetic variations or mutations in the gene encoding AFM protein may impact its expression or function, leading to altered cellular phenotypes or disease susceptibility, and these can be investigated through genetic association studies or functional characterization of mutant variants.
AFM protein influences cellular biomechanics, and its effects on cell stiffness, contractility, or cytoskeletal organization can be investigated through techniques like atomic force microscopy, traction force microscopy, or cytoskeletal staining.
Dysregulation or dysfunction of AFM protein contributes to pathologies or disease conditions, and associations can be investigated using disease models, patient samples, or genetically modified systems, combined with phenotypic assessments or molecular analysis techniques.
Customer Reviews (3)
Write a reviewProbing protein-protein interactions in chromatin modifications for gene regulation.
Deciphering protein-protein interactions in mitochondrial function for cellular energy production.
Studying protein-protein interactions in apoptosis pathways for programmed cell death.
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