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Recombinant Cynomolgus CD63 cell lysate

Cat.No. : CD63-814CCL
Product Overview : Cynomolgus CD63 / Tspan-30 / Tetraspanin-30 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Cynomolgus
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : CD63 CD63 molecule [ Macaca fascicularis ]
Official Symbol : CD63
Synonyms : CD63 molecule
Gene ID : 102132387
mRNA Refseq : XM_005571130
Protein Refseq : XP_005571187
Chromosome Location : chromosome: 11

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How is CD63 involved in cancer progression? 08/12/2022

It is associated with cancer progression, impacting cell migration and metastasis.

What role does CD63 play in immune cell function? 01/27/2021

CD63 modulates immune responses, influencing antigen presentation and cell activation.

Are there potential therapeutic applications targeting CD63? 10/03/2020

Targeting CD63 may offer therapeutic potential in cancer and immune-related diseases.

How is CD63 expression regulated in cells? 05/12/2020

Its expression is regulated through transcriptional and post-translational mechanisms.

How does CD63 contribute to vesicular trafficking? 01/02/2019

It helps in the sorting and transport of proteins within endosomes and lysosomes.

What is the relationship between CD63 and exosomes? 08/07/2018

CD63 is a marker for exosomes, playing a role in their formation and function.

What is the primary function of CD63 in cellular processes? 01/18/2018

CD63 is involved in vesicular trafficking, cell adhesion, and signaling.

Customer Reviews (3)

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Reviews
11/26/2022

    Consistent and reliable protein.

    08/23/2022

      Excellent yield, very pure.

      06/19/2021

        Perfect for specific experiments.

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