Recombinant Mouse SELE cell lysate
Cat.No. : | SELE-845MCL |
Product Overview : | Mouse SELE / selectin E / CD62E derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
- Related Products
Source : | Human cells |
Species : | Mouse |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | Sele selectin, endothelial cell [ Mus musculus ] |
Official Symbol : | SELE |
Synonyms : | SELE; selectin, endothelial cell; E-selectin; ELAM-1; LECAM2; CD62 antigen-like family member E; endothelial leukocyte adhesion molecule 1; leukocyte-endothelial cell adhesion molecule 2; Elam; CD62E; |
Gene ID : | 20339 |
mRNA Refseq : | NM_011345 |
Protein Refseq : | NP_035475 |
Pathway : | African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Malaria, organism-specific biosystem; Malaria, conserved biosystem; |
Function : | binding; oligosaccharide binding; phospholipase binding; sialic acid binding; sugar binding; transmembrane signaling receptor activity; |
Products Types
◆ Recombinant Protein | ||
SELE-210H | Recombinant Human SELE Protein, Fc-tagged | +Inquiry |
SELE-05H | Active Recombinant Human SELE Protein, His-tagged | +Inquiry |
SELE-128H | Recombinant Human SELE Protein, His-tagged | +Inquiry |
Sele-6390R | Recombinant Rat Sele Protein, His (Fc)-Avi-tagged | +Inquiry |
CD62E-42H | Recombinant Human CD62E Protein, C-8His tagged, Biotinylated | +Inquiry |
◆ Lysates | ||
SELE-960RCL | Recombinant Rat SELE cell lysate | +Inquiry |
SELE-2992HCL | Recombinant Human SELE cell lysate | +Inquiry |
SELE-1099CCL | Recombinant Cynomolgus SELE cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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