Recombinant Cynomolgus PTGFRN cell lysate
Cat.No. : | PTGFRN-856CCL |
Product Overview : | Cynomolgus EWI-F / PTGFRN derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Cynomolgus |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | PTGFRN prostaglandin F2 receptor inhibitor [ Macaca fascicularis ] |
Official Symbol : | PTGFRN |
Synonyms : | prostaglandin F2 receptor inhibitor |
Gene ID : | 102120418 |
mRNA Refseq : | XM_005542200 |
Protein Refseq : | XP_005542257 |
Chromosome Location : | chromosome: 1 |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionGenetic mutations in PTGFRN can lead to disrupted cell communication and adhesion, affecting overall cell function.
PTGFRN, a cell membrane protein, is crucial for facilitating communication between cells.
PTGFRN works with other membrane proteins to regulate cell interactions and signaling pathways.
Targeting PTGFRN could offer new strategies for treatments focusing on cell communication and adhesion.
It plays a key role in cell adhesion, maintaining cell structure and interactions.
PTGFRN is involved in transmitting signals across the cell membrane, affecting cellular responses.
Altered PTGFRN activity can contribute to various diseases due to its role in cell signaling and structure.
Customer Reviews (3)
Write a reviewPrecision in analysis, critical for our research integrity.
Efficient and precise, integral to experiments.
Enhances research credibility, saves time and effort.
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