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Recombinant Cynomolgus PTGFRN cell lysate

Cat.No. : PTGFRN-856CCL
Product Overview : Cynomolgus EWI-F / PTGFRN derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Cynomolgus
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : PTGFRN prostaglandin F2 receptor inhibitor [ Macaca fascicularis ]
Official Symbol : PTGFRN
Synonyms : prostaglandin F2 receptor inhibitor
Gene ID : 102120418
mRNA Refseq : XM_005542200
Protein Refseq : XP_005542257
Chromosome Location : chromosome: 1

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How do genetic variations in PTGFRN affect cell function? 08/29/2022

Genetic mutations in PTGFRN can lead to disrupted cell communication and adhesion, affecting overall cell function.

What is the primary role of PTGFRN in cell communication? 07/12/2022

PTGFRN, a cell membrane protein, is crucial for facilitating communication between cells.

How does PTGFRN interact with other cell membrane proteins? 12/24/2021

PTGFRN works with other membrane proteins to regulate cell interactions and signaling pathways.

What potential therapeutic applications arise from targeting PTGFRN in medical treatments? 09/09/2021

Targeting PTGFRN could offer new strategies for treatments focusing on cell communication and adhesion.

How does PTGFRN contribute to cell adhesion? 10/24/2020

It plays a key role in cell adhesion, maintaining cell structure and interactions.

What role does PTGFRN play in signal transduction? 12/30/2017

PTGFRN is involved in transmitting signals across the cell membrane, affecting cellular responses.

What is the impact of altered PTGFRN activity on disease development? 07/07/2017

Altered PTGFRN activity can contribute to various diseases due to its role in cell signaling and structure.

Customer Reviews (3)

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Reviews
10/01/2021

    Precision in analysis, critical for our research integrity.

    12/29/2019

      Efficient and precise, integral to experiments.

      05/04/2017

        Enhances research credibility, saves time and effort.

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