"NRP2" Related Products

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Recombinant Human NRP2 cell lysate

Cat.No.: NRP2-937HCL
Product Overview: Human Neuropilin 2 / NRP2 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
Source: Human cells
Species: Human
Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability: Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name: NRP2 neuropilin 2 [ Homo sapiens ]
Official Symbol: NRP2
Synonyms: NRP2; neuropilin 2; neuropilin-2; VEGF165R2; neuropilin-2b(0); neuropilin-2a(17); neuropilin-2a(22); receptor for VEGF165 and semaphorins class3; vascular endothelial cell growth factor 165 receptor 2; NP2; NPN2; PRO2714; MGC126574;
Gene ID: 8828
mRNA Refseq: NM_003872
Protein Refseq: NP_003863
MIM: 602070
UniProt ID: O60462
Chromosome Location: 2q33.3
Pathway: Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; L1CAM interactions, organism-specific biosystem; Neurophilin interactions with VEGF and VEGFR, organism-specific biosystem; NrCAM interactions, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by VEGF, organism-specific biosystem;
Function: cytokine binding; growth factor binding; heparin binding; metal ion binding; receptor activity; semaphorin receptor activity; vascular endothelial growth factor-activated receptor activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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