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Recombinant Cynomolgus SELE cell lysate

Cat.No. : SELE-1099CCL
Product Overview : Cynomolgus SELE / selectin E derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer. Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Cynomolgus
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : SELE selectin E [ Macaca fascicularis ]
Official Symbol : SELE
Synonyms : selectin E
Gene ID : 102143564
mRNA Refseq : XM_005539941
Protein Refseq : XP_005539998
Chromosome Location : chromosome: 1
Pathway : African trypanosomiasis, organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; TNF signaling pathway, organism-specific biosystem

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Is there a diagnostic test to measure SELE levels in patients? 11/30/2021

Some diagnostic tests can measure SELE levels in the blood, providing insights into the inflammatory status of an individual.

Can SELE protein be involved in cancer progression? 01/18/2020

Emerging research suggests a potential role of SELE in cancer metastasis, making it a target for investigation in cancer therapy.

Are there any genetic factors influencing SELE expression? 01/05/2018

Genetic variations can influence SELE expression, contributing to individual differences in inflammatory responses.

How does SELE protein interact with circulating immune cells? 01/09/2017

SELE binds to specific receptors on immune cells, facilitating their adhesion to endothelial cells and promoting their migration to inflammatory sites.

Can targeting SELE protein be a potential strategy for anti-inflammatory therapies? 08/05/2016

Yes, drugs designed to modulate SELE activity are being explored as potential anti-inflammatory agents.

Customer Reviews (3)

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Reviews
11/26/2020

    With its remarkable purity and reliability, coupled with the manufacturer's excellent technical support, this protein is a crucial asset in addressing my scientific inquiries and advancing my research goals.

    09/08/2019

      In addition to its outstanding quality, the SELE Protein exhibits exceptional stability during storage and usage.

      06/16/2019

        Its ability to maintain structural integrity and stability ensures accurate imaging, enabling me to gain deeper insights into the three-dimensional organization and interactions of the proteins under investigation.

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