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Recombinant Rat ADAM17 cell lysate

Cat.No. : ADAM17-1198RCL
Product Overview : Rat ADAM17 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Rat
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Adam17 ADAM metallopeptidase domain 17 [ Rattus norvegicus ]
Official Symbol : ADAM17
Synonyms : ADAM17; ADAM metallopeptidase domain 17; disintegrin and metalloproteinase domain-containing protein 17; ADAM 17; TNF-alpha convertase; TNF-alpha converting enzyme; TNF-alpha-converting enzyme; a disintegrin and metalloprotease domain 17; a disintegrin and metallopeptidase domain 17; a disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme); TACE;
Gene ID : 57027
mRNA Refseq : NM_020306
Protein Refseq : NP_064702
Pathway : Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Delta-Notch Signaling Pathway, organism-specific biosystem; Disease, organism-specific biosystem; Notch signaling pathway, organism-specific biosystem; Notch signaling pathway, conserved biosystem; Regulated proteolysis of p75NTR, organism-specific biosystem;
Function : PDZ domain binding; PDZ domain binding; SH3 domain binding; integrin binding; integrin binding; interleukin-6 receptor binding; interleukin-6 receptor binding; metal ion binding; metalloendopeptidase activity; metalloendopeptidase activity; metalloendopeptidase activity; metallopeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Is ADAM17 protein involved in the renin-angiotensin system? 08/01/2022

ADAM17 is a negative regulator of the RAS system.

My research requires high protein purity, can I customize > 90% ADAM17 protein? 06/05/2022

Yes, we have produced ADAM17 protein of this purity.

What proteins does ADAM17 cleave? 04/07/2022

ADAM17 protein can cleave IL6R, TNFR1, ACE2, etc.

Where ADAM17 is highly expressed? 06/25/2021

ADAM17 is widely expressed in somatic and immune cells

In addition to Diabetes mellitus and cardiomyopathy-related research, ADAM17 is currently used in the research of which diseases? 01/21/2021

ADAM17 is also currently being used in studies of breast cancer and gastric cancer, etc.

Is ADAM17 associated with the EGFR-PI3K-AKT pathway? 10/03/2020

ADAM17 is an activator that mediates the EGFR-PI3K-AKT pathway.

Does the package insert for ADAM17 contain detailed information on the handling of the lyophilized powder product? 04/19/2020

The product manual will contain relevant content on product handling, and you can also consult our technical experts on related issues.

ADAM17 protein is also involved in recent activities? 12/11/2019

Yes, ADAM17 protein is involved in this promotion.

Does ADAM17 mediate the Notch pathway? 10/05/2019

ADAM17 acts as an activator of Notch pathway by mediating the of cleavage of Notch.

Does adding His tag have any effect on protein? 04/24/2019

Addition of His tags usually does not affect the function, properties of the target protein.

Customer Reviews (3)

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Reviews
04/08/2022

    Cheap price, high quality products, worth buying again

    03/15/2019

      The high quality of the purchased protein helped me to obtain satisfactory experimental results.

      02/26/2019

        Good after-sales service attitude, helped me solve a lot of problems in the experiment.

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