Recombinant Rat CD40LG cell lysate
Cat.No. : | CD40LG-1205RCL |
Product Overview : | Rat CD40LG / CD40L / TNFSF5 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Rat |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | Cd40lg CD40 ligand [ Rattus norvegicus ] |
Official Symbol : | CD40LG |
Synonyms : | CD40LG; CD40 ligand; CD40-L; CD40 ligand CD154; tumor necrosis factor ligand superfamily member 5; tumor necrosis factor (ligand) superfamily, member 5; Cd40l; Tnfsf5; |
Gene ID : | 84349 |
mRNA Refseq : | NM_053353 |
Protein Refseq : | NP_445805 |
Pathway : | Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; |
Function : | CD40 receptor binding; CD40 receptor binding; CD40 receptor binding; cytokine activity; molecular_function; tumor necrosis factor receptor binding; |
Products Types
◆ Recombinant Protein | ||
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CD40LG-323H | Active Recombinant Human CD40LG Protein (Met1-Leu261), C-His tagged, Animal-free, Carrier-free | +Inquiry |
CD40LG-0816H | Recombinant Human CD40LG Protein, His-Flag-StrepII-Tagged | +Inquiry |
CD40LG-197H | Recombinant Human CD40LG Protein, C-His-tagged | +Inquiry |
◆ Lysates | ||
CD40LG-1548MCL | Recombinant Mouse CD40LG cell lysate | +Inquiry |
CD40LG-1080CCL | Recombinant Cynomolgus CD40LG cell lysate | +Inquiry |
CD40LG-1965HCL | Recombinant Human CD40LG cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
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Q&As (5)
Ask a questionYes, there are clinical trials investigating the modulation of CD40LG in autoimmune diseases, exploring its potential role in regulating immune responses that contribute to autoimmunity.
Yes, research suggests that CD40LG modulation might be a viable strategy in the development of treatments for infectious diseases by enhancing immune responses.
The interaction between CD40LG and CD40 is crucial for the activation of B cells, leading to the production of antibodies and the development of an effective immune response.
CD40LG is involved in modulating inflammatory responses by influencing the activation and function of immune cells, contributing to the regulation of the immune system.
CD40LG is being investigated as an adjuvant in vaccine development, aiming to enhance the immune response and improve the efficacy of vaccines against various diseases.
Customer Reviews (3)
Write a reviewThe reliable performance of the CD40LG protein in ELISA and its compatibility with protein electron microscopy structure analysis make it an excellent choice for a wide range of research studies.
Its reliable and accurate performance ensures dependable results, contributing to the advancement of scientific knowledge in various fields of study.
Their knowledgeable and responsive team assists in troubleshooting and finding solutions to any experimental challenges that may arise.
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