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Recombinant Human RNASE1 cell lysate

Cat.No. : RNASE1-1283HCL
Product Overview : Human RNase A / Ribonuclease A / RNASE1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : RNASE1 ribonuclease, RNase A family, 1 (pancreatic) [ Homo sapiens ]
Official Symbol : RNASE1
Synonyms : RNASE1; ribonuclease, RNase A family, 1 (pancreatic); RNS1; ribonuclease pancreatic; RIB-1; RNase 1; RNase A; HP-RNase; RNase upI-1; ribonuclease 1; ribonuclease A; RIB1; MGC12408;
Gene ID : 6035
mRNA Refseq : NM_002933
Protein Refseq : NP_002924
MIM : 180440
UniProt ID : P07998
Chromosome Location : 14q11.1
Function : endonuclease activity; hydrolase activity; nucleic acid binding; pancreatic ribonuclease activity; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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Is there an association between RNASE1 protein and insulin secretion? 11/12/2019

There is a correlation between RNASE1 protein and insulin secretion. Studies have shown that RNASE1 protein can regulate insulin synthesis and secretion by affecting the internal environment of pancreatic islets.

Is there a regulatory mechanism for RNASE1 proteins? 10/23/2019

At present, the regulatory mechanism of RNASE1 protein is poorly understood. However, studies have found that some factors such as glucagon and insulin may be involved in regulating the expression and activity of RNASE1 protein.

Does RNASE1 protein have anti-tumor effects? 07/30/2019

The RNASE1 protein is thought to have potential for anti-tumor effects. Studies have shown that it can induce apoptosis of tumor cells and inhibit the proliferation and invasion ability of tumor cells.

What are the applications of RNASE1 protein in clinical diagnosis? 07/15/2019

RNASE1 protein has certain application potential in clinical diagnosis. Its hydrolyzed RNA properties can be used to measure the content and stability of RNA, and then evaluate the condition and treatment effect of certain diseases.

What role does RNASE1 protein play in RNA degradation? 04/23/2019

The RNASE1 protein participates in the RNA degradation process in the digestive system by cutting the phosphodiester bond of the RNA molecule and degrading it into shorter RNA fragments.

Is RNASE1 protein also expressed in the gut? 01/19/2019

The main expression and secretion site of RNASE1 protein is the pancreas, while the expression in the gut is relatively low. But in some cases, other nucleases in the gut may perform a similar function.

Customer Reviews (3)

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Reviews
10/27/2022

    RNASE1 have strong anti-contamination ability and are not easily contaminated, which ensures the quality of the product.

    07/06/2021

      This stability of RNASE1 is impressive, maintaining stable performance regardless of the environment, demonstrating excellent quality and reliability.

      01/08/2020

        The catalytic activity of RNASE1 is excellent, which can simplify complex chemical reactions and improve yields, and has high scientific research value.

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