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Recombinant Mouse SEMA5A cell lysate

Cat.No. : SEMA5A-1310MCL
Product Overview : Mouse SEMA5A / Semaphorin-5A / SEMAF derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Sema5a sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A [ Mus musculus ]
Official Symbol : SEMA5A
Synonyms : SEMA5A; sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A; semaphorin-5A; sema F; M-Sema D; semaphorin F; semaphorin-F; semF; Semaf; AI464145; 5930434A13; 9130201M22Rik;
Gene ID : 20356
mRNA Refseq : NM_009154
Protein Refseq : NP_033180
Pathway : Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Other semaphorin interactions, organism-specific biosystem; Semaphorin interactions, organism-specific biosystem;
Function : axon guidance receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What is the primary function of Semaphorin-5A (SEMA5A) in cellular processes and neural development? 06/10/2021

Semaphorin-5A (SEMA5A) functions in cellular processes, primarily playing a role in neural development and axon guidance.

In which cellular compartments is SEMA5A predominantly localized, especially within the nervous system? 11/27/2019

SEMA5A is predominantly localized in growth cones and axonal pathways within the nervous system.

Are there therapeutic implications or potential targets related to SEMA5A in neurological disorders involving neural development? 08/10/2018

There are potential therapeutic implications related to SEMA5A in neurological disorders involving neural development, making it a target for drug development.

How does SEMA5A participate in axon guidance and neuronal migration during neural development? 05/25/2018

SEMA5A participates in axon guidance and neuronal migration during neural development by interacting with Plexin-A and Neuropilin-1 receptors.

Are there specific receptors or co-receptors that SEMA5A interacts with, influencing its role in neural circuit formation? 02/27/2018

Specific receptors or co-receptors that SEMA5A interacts with include Plexin-A and Neuropilin-1, influencing its role in neural circuit formation.

How does SEMA5A contribute to the regulation of synaptic connectivity and plasticity in the nervous system? 09/05/2016

SEMA5A contributes to the regulation of synaptic connectivity and plasticity in the nervous system, impacting neural circuit formation.

What diseases or conditions are associated with dysregulation or abnormal expression of SEMA5A? 05/11/2016

Dysregulation or abnormal expression of SEMA5A is associated with conditions such as autism spectrum disorders and developmental disorders.

Customer Reviews (3)

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Reviews
11/15/2022

    The product's consistent outcomes fortified the reproducibility of our scientific inquiries.

    03/26/2021

      Despite its cost, the product's contributions to our research goals made it an invaluable asset.

      03/09/2019

        We appreciated the product's efficiency in achieving target protein upregulation with minimal time investment.

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