Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human AZGP1 cell lysate

Cat.No. : AZGP1-1342HCL
Product Overview : Human Alpha-2-glycoprotein / AZGP1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
  • Specification
  • Gene Information
  • Related Products
Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : AZGP1 alpha-2-glycoprotein 1, zinc-binding [ Homo sapiens ]
Official Symbol : AZGP1
Synonyms : AZGP1; alpha-2-glycoprotein 1, zinc-binding; alpha 2 glycoprotein 1, zinc; zinc-alpha-2-glycoprotein; ZA2G; ZAG; zn-alpha-2-GP; Zn-alpha2-glycoprotein; zn-alpha-2-glycoprotein; Alpha-2-glycoprotein, zinc;
Gene ID : 563
mRNA Refseq : NM_001185
Protein Refseq : NP_001176
MIM : 194460
UniProt ID : P25311
Chromosome Location : 7q22.1
Function : fatty acid binding; protein transmembrane transporter activity; ribonuclease activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (5)

Ask a question
Is there a connection between AZGP1 and metabolic disorders? 09/15/2022

Yes, AZGP1 is implicated in metabolic disorders, and its study may offer insights into the development of therapies for conditions like obesity and diabetes.

Where is AZGP1 protein primarily found in the human body? 09/29/2020

AZGP1 is primarily found in various tissues, including the liver, adipose tissue, and prostate.

Does AZGP1 play a role in inflammation or immune response? 05/13/2017

Some studies suggest that AZGP1 may have immunomodulatory properties, and its role in inflammation is an area of active investigation.

AZGP1 is involved in lipid metabolism and has been linked to the regulation of adipose tissue and energy homeostasis. 12/26/2016

AZGP1 is involved in lipid metabolism and has been linked to the regulation of adipose tissue and energy homeostasis.

How does AZGP1 protein relate to adipose tissue and metabolism? 12/20/2016

AZGP1 is involved in lipid metabolism and has been linked to the regulation of adipose tissue and energy homeostasis.

Customer Reviews (3)

Write a review
Reviews
08/25/2021

    What further sets the AZGP1 Protein apart is the outstanding technical support provided by the manufacturer.

    07/07/2019

      Considering the aforementioned qualities, I wholeheartedly recommend the AZGP1 Protein to fellow researchers in search of a top-tier protein solution that not only meets their experimental needs but also benefits from exceptional technical support.

      10/08/2017

        By incorporating the AZGP1 Protein into their studies, scientists have the opportunity to conduct their research with utmost confidence, paving the way for groundbreaking discoveries and novel insights in their respective fields.

        Ask a Question for All AZGP1 Products

        Required fields are marked with *

        My Review for All AZGP1 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends