Recombinant Rat TNFSF15 cell lysate
Cat.No. : | TNFSF15-1386RCL |
Product Overview : | Rat TNFSF15 / TL1A derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Rat |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | Tnfsf15 tumor necrosis factor (ligand) superfamily, member 15 [ Rattus norvegicus ] |
Official Symbol : | TNFSF15 |
Synonyms : | TNFSF15; tumor necrosis factor (ligand) superfamily, member 15; tumor necrosis factor ligand superfamily member 15; TNF ligand-related molecule 1; vascular endothelial cell growth inhibitor; |
Gene ID : | 252878 |
mRNA Refseq : | NM_145765 |
Protein Refseq : | NP_665708 |
Pathway : | Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; |
Function : | cytokine activity; death receptor binding; tumor necrosis factor receptor binding; |
Products Types
◆ Recombinant Protein | ||
TNFSF15-17H | Recombinant Human TNFSF15 Protein, N-terminal His and AviTag™, Biotinylated | +Inquiry |
TNFSF15-5860R | Recombinant Rat TNFSF15 Protein, His (Fc)-Avi-tagged | +Inquiry |
TNFSF15-150H | Recombinant Human TNFSF15 Trimer protein, N-His-Flag-tagged | +Inquiry |
TNFSF15-326H | Active Recombinant Human TNFSF15 Protein (Gln58-Leu251), C-His tagged, Animal-free, Carrier-free | +Inquiry |
TNFSF15-556H | Recombinant Human TNFSF15 Protein, Fc-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionTNFSF15 interacts with receptors on immune cells, influencing their activity and contributing to the overall regulation of immune responses.
Several clinical trials are underway to evaluate the efficacy and safety of therapies targeting TNFSF15 in autoimmune diseases.
TNFSF15 levels may serve as a biomarker for certain autoimmune conditions, aiding in the diagnosis and monitoring of disease progression.
TNFSF15 is considered a promising target for drug development, and ongoing research aims to identify compounds that modulate its activity effectively.
In IBD, TNFSF15 is believed to be involved in the inflammation process, contributing to the chronic nature of the disease.
Customer Reviews (3)
Write a reviewThey keep up with the latest research findings and continually refine their product based on customer feedback and emerging scientific trends.
Their prompt assistance and prompt response can swiftly resolve any challenges, enabling smooth experimentation and maintaining the integrity of the results.
Their knowledgeable support team is well-equipped to address specific inquiries, suggest experimental protocols, and provide guidance throughout the entire research process.
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