Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Rat TNFSF15 cell lysate

Cat.No. : TNFSF15-1386RCL
Product Overview : Rat TNFSF15 / TL1A derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
  • Specification
  • Gene Information
  • Related Products
Source : Human cells
Species : Rat
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Tnfsf15 tumor necrosis factor (ligand) superfamily, member 15 [ Rattus norvegicus ]
Official Symbol : TNFSF15
Synonyms : TNFSF15; tumor necrosis factor (ligand) superfamily, member 15; tumor necrosis factor ligand superfamily member 15; TNF ligand-related molecule 1; vascular endothelial cell growth inhibitor;
Gene ID : 252878
mRNA Refseq : NM_145765
Protein Refseq : NP_665708
Pathway : Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem;
Function : cytokine activity; death receptor binding; tumor necrosis factor receptor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (5)

Ask a question
How does TNFSF15 interact with other components of the immune system? 11/09/2020

TNFSF15 interacts with receptors on immune cells, influencing their activity and contributing to the overall regulation of immune responses.

Are there any current clinical trials targeting TNFSF15 for autoimmune disease treatments? 07/31/2017

Several clinical trials are underway to evaluate the efficacy and safety of therapies targeting TNFSF15 in autoimmune diseases.

Are there any diagnostic implications of TNFSF15 in autoimmune disorders? 01/07/2017

TNFSF15 levels may serve as a biomarker for certain autoimmune conditions, aiding in the diagnosis and monitoring of disease progression.

Can TNFSF15 be a potential target for novel drug development in autoimmune diseases? 12/06/2016

TNFSF15 is considered a promising target for drug development, and ongoing research aims to identify compounds that modulate its activity effectively.

How does TNFSF15 contribute to the pathogenesis of inflammatory bowel disease (IBD)? 05/24/2016

In IBD, TNFSF15 is believed to be involved in the inflammation process, contributing to the chronic nature of the disease.

Customer Reviews (3)

Write a review
Reviews
03/21/2022

    They keep up with the latest research findings and continually refine their product based on customer feedback and emerging scientific trends.

    05/01/2021

      Their prompt assistance and prompt response can swiftly resolve any challenges, enabling smooth experimentation and maintaining the integrity of the results.

      10/01/2017

        Their knowledgeable support team is well-equipped to address specific inquiries, suggest experimental protocols, and provide guidance throughout the entire research process.

        Ask a Question for All TNFSF15 Products

        Required fields are marked with *

        My Review for All TNFSF15 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends