Recombinant Mouse S100B cell lysate
Cat.No. : | S100B-1418MCL |
Product Overview : | Mouse S100B / S100beta derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Mouse |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | S100b S100 protein, beta polypeptide, neural [ Mus musculus ] |
Official Symbol : | S100B |
Synonyms : | S100B; S100 protein, beta polypeptide, neural; protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B; Bpb; AI850290; MGC74317; |
Gene ID : | 20203 |
mRNA Refseq : | NM_009115 |
Protein Refseq : | NP_033141 |
Pathway : | Activated TLR4 signalling, organism-specific biosystem; Cytosolic sensors of pathogen-associated DNA, organism-specific biosystem; DAI mediated induction of type I IFNs, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; MyD88 cascade initiated on plasma membrane, organism-specific biosystem; MyD88 dependent cascade initiated on endosome, organism-specific biosystem; |
Function : | RAGE receptor binding; S100 beta binding; calcium ion binding; calcium ion binding; calcium-dependent protein binding; identical protein binding; metal ion binding; protein homodimerization activity; receptor binding; zinc ion binding; |
Products Types
◆ Recombinant Protein | ||
S100B-68H | Active Recombinant Human S100B protein(Ser2-Glu92), hFc-tagged | +Inquiry |
S100B-4876R | Recombinant Rat S100B Protein, His (Fc)-Avi-tagged | +Inquiry |
S100B-2542H | Recombinant Human S100B protein(11-80 aa), C-His-tagged | +Inquiry |
S100B-0052H | Recombinant Human S100B Protein | +Inquiry |
S100B-042H | Recombinant Human S100B Protein, MYC/DDK-tagged, C13 and N15-labeled | +Inquiry |
◆ Native Protein | ||
S100B-1116H | Native Human S100B Protein | +Inquiry |
S100B-257B | Native Bovine S-100b Protein | +Inquiry |
S100B-256B | Native Bovine S-100 Protein | +Inquiry |
◆ Lysates | ||
S100B-2858HCL | Recombinant Human S100B cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionElevated S100B levels in the blood can indicate TBI, and its measurement can aid in TBI diagnosis and severity assessment.
S100B can be used to diagnose brain injuries, such as traumatic brain injury and stroke.
S100B levels are typically measured through a blood test.
Results should be interpreted in the context of the patient's clinical history and other diagnostic tests.
S100B may help in monitoring disease progression and severity in conditions like Alzheimer's disease.
Customer Reviews (3)
Write a reviewOne major advantage of S100B protein is its ability to contribute to the understanding of diseases related to aberrant cell cycle regulation, including cancer.
The S100B protein demonstrates exceptional quality, making it an ideal candidate to fulfill my experimental requirements.
The manufacturer of S100B protein plays a crucial role in supporting my research efforts.
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