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Recombinant Mouse S100B cell lysate

Cat.No. : S100B-1418MCL
Product Overview : Mouse S100B / S100beta derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
  • Specification
  • Gene Information
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : S100b S100 protein, beta polypeptide, neural [ Mus musculus ]
Official Symbol : S100B
Synonyms : S100B; S100 protein, beta polypeptide, neural; protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B; Bpb; AI850290; MGC74317;
Gene ID : 20203
mRNA Refseq : NM_009115
Protein Refseq : NP_033141
Pathway : Activated TLR4 signalling, organism-specific biosystem; Cytosolic sensors of pathogen-associated DNA, organism-specific biosystem; DAI mediated induction of type I IFNs, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; MyD88 cascade initiated on plasma membrane, organism-specific biosystem; MyD88 dependent cascade initiated on endosome, organism-specific biosystem;
Function : RAGE receptor binding; S100 beta binding; calcium ion binding; calcium ion binding; calcium-dependent protein binding; identical protein binding; metal ion binding; protein homodimerization activity; receptor binding; zinc ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How does S100B help in the diagnosis of traumatic brain injury (TBI)? 01/29/2023

Elevated S100B levels in the blood can indicate TBI, and its measurement can aid in TBI diagnosis and severity assessment.

What conditions can S100B be used to diagnose? 01/11/2023

S100B can be used to diagnose brain injuries, such as traumatic brain injury and stroke.

How is S100B measured in clinical settings? 02/24/2020

S100B levels are typically measured through a blood test.

What precautions should be taken when interpreting S100B results? 02/02/2017

Results should be interpreted in the context of the patient's clinical history and other diagnostic tests.

How is S100B used in the context of neurodegenerative diseases? 02/07/2016

S100B may help in monitoring disease progression and severity in conditions like Alzheimer's disease.

Customer Reviews (3)

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Reviews
03/15/2022

    One major advantage of S100B protein is its ability to contribute to the understanding of diseases related to aberrant cell cycle regulation, including cancer.

    09/29/2019

      The S100B protein demonstrates exceptional quality, making it an ideal candidate to fulfill my experimental requirements.

      10/23/2018

        The manufacturer of S100B protein plays a crucial role in supporting my research efforts.

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