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Recombinant Mouse RETN cell lysate

Cat.No. : RETN-1635MCL
Product Overview : Mouse Resistin / ADSF / RETN derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Retn resistin [ Mus musculus ]
Official Symbol : RETN
Synonyms : RETN; resistin; found in inflammatory zone 3; cysteine-rich secreted protein FIZZ3; adipose tissue-specific secretory factor; dominant inhibitory adipocyte-specific secretory factor; adipose-specific cysteine-rich secreted protein A12-alpha; ADSF; Rstn; Xcp4; Fizz3;
Gene ID : 57264
mRNA Refseq : NM_001204959
Protein Refseq : NP_001191888
Pathway : Adipogenesis, organism-specific biosystem;
Function : hormone activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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How does the expression level of RETN correlate with insulin resistance? 11/03/2022

Expression level of RETN is associated with the development of insulin resistance. High levels of RETN can reduce insulin sensitivity, leading to decreased glycemic control, which promotes the development of insulin resistance.

What is the role of RETN in the treatment of diabetes and cardiovascular disease? 08/13/2022

Studies have shown that regulating the expression level of RETN can influence the development and treatment effect of diabetes and cardiovascular disease. For example, certain drugs can improve insulin sensitivity and control blood sugar levels by inhibiting the expression level of RETN.

How can the expression level of RETN be used for the prediction and diagnosis of diabetes and cardiovascular disease? 05/11/2022

Diabetes and cardiovascular disease can be predicted and diagnosed by measuring the expression level of RETN. For example, high levels of RETN may indicate a higher risk of disease and more severe disease conditions.

How to use mutation or abnormal expression of RETN for disease diagnosis and prognosis assessment? 12/21/2021

Mutations and expression levels of the RETN gene can be detected to aid in diagnosing disease and assessing prognosis. For example, certain mutations may predict a higher risk and a worse prognosis.

What diseases are mutations or abnormal expression of RETN associated with? 04/22/2020

Mutations or abnormal expression of RETN may be associated with a variety of diseases. For example, certain mutations can lead to abnormally elevated levels of their expression, increasing the risk of diabetes, cardiovascular disease, etc.

How does RETN relate to other biomarkers? 05/16/2019

RETN has some association with other biomarkers. For example, in obesity and cardiovascular disease, the expression level of RETN can be used to assess the prognosis and therapeutic effect of the disease together with biomarkers such as adiponectin and leptin.

Customer Reviews (3)

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Reviews
06/22/2020

    Catalytic activity of RETN is excellent, no matter what the environment can maintain stable catalytic performance, its reliability is reliable.

    05/27/2020

      The quality of RETN they produce is guaranteed, and we can buy it with confidence.

      05/22/2019

        The stability of RETN is too strong to ignore, and it remains admirably stable after long storage.

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