Recombinant Mouse CD55 cell lysate
Cat.No. : | CD55-1669MCL |
Product Overview : | Mouse CD55 / DAF derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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- Gene Information
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Source : | Human cells |
Species : | Mouse |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | Cd55 CD55 antigen [ Mus musculus ] |
Official Symbol : | CD55 |
Synonyms : | CD55; CD55 antigen; complement decay-accelerating factor, GPI-anchored; Cromer blood group; GPI anchor addition signal; decay accelerating factor 1; complement-glycosylphosphatidylinositol; Daf; Daf1; Daf-GPI; GPI-DAF; |
Gene ID : | 13136 |
mRNA Refseq : | NM_010016 |
Protein Refseq : | NP_034146 |
Pathway : | Complement Activation, Classical Pathway, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Viral myocarditis, organism-specific biosystem; |
Function : | enzyme inhibitor activity; |
Products Types
◆ Recombinant Protein | ||
Cd55-6893M | Recombinant Mouse Cd55 protein(Met1-Thr361), His-tagged | +Inquiry |
CD55-01H | Recombinant Human CD55 Protein (35-353aa), C-His-tagged | +Inquiry |
CD55-2325H | Recombinant Human CD55 Protein, GST-tagged | +Inquiry |
CD55-689H | Recombinant Human CD55 Protein, His-tagged | +Inquiry |
CD55-64H | Active Recombinant Human CD55 protein(Met1-Ser353), hFc-tagged | +Inquiry |
◆ Lysates | ||
CD55-2531HCL | Recombinant Human CD55 cell lysate | +Inquiry |
CD55-1433RCL | Recombinant Rat CD55 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionGenetic variations in CD55 may influence susceptibility to complement-mediated disorders by affecting the protein's ability to regulate complement activation. This can lead to conditions like paroxysmal nocturnal hemoglobinuria (PNH).
CD55 has immunomodulatory functions beyond complement regulation, influencing T cell responses and contributing to the maintenance of immune tolerance.
CD55 protects cells from complement-mediated lysis by preventing the formation of membrane attack complexes. This contributes to immune evasion by pathogens and self-cells alike.
CD55 consists of extracellular domains responsible for interactions with complement proteins, particularly C3b and C4b. These interactions prevent the formation of membrane attack complexes on host cells.
CD55 dysregulation is associated with autoimmune disorders, where it may contribute to immune system overactivity. Additionally, some pathogens exploit CD55 to evade host immune responses.
Customer Reviews (3)
Write a reviewEfficient shipping and exceptional product quality. This company knows how to serve its customers.
Consistency is key in research, and this product delivers that every time.
We've tried various proteins, but this one stands out for its purity and performance.
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