Recombinant Mouse TFRC cell lysate
Cat.No. : | TFRC-1813MCL |
Product Overview : | Mouse TFRC / CD71 / Transferrin Receptor derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Mouse |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | Tfrc transferrin receptor [ Mus musculus ] |
Official Symbol : | TFRC |
Synonyms : | TFRC; transferrin receptor; transferrin receptor protein 1; mammary tumor virus receptor 1; TR; TFR; p90; CD71; TFR1; Trfr; Mtvr1; Mtvr-1; AI195355; AI426448; AU015758; 2610028K12Rik; E430033M20Rik; |
Gene ID : | 22042 |
mRNA Refseq : | NM_011638 |
Protein Refseq : | NP_035768 |
Pathway : | Clathrin derived vesicle budding, organism-specific biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; Golgi Associated Vesicle Biogenesis, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Membrane Trafficking, organism-specific biosystem; |
Function : | Hsp70 protein binding; chaperone binding; iron ion transmembrane transporter activity; peptidase activity; receptor activity; transferrin receptor activity; transferrin receptor activity; |
Products Types
◆ Recombinant Protein | ||
TFRC-2532H | Recombinant Human TFRC protein(91-170 aa), C-His-tagged | +Inquiry |
TFRC-466H | Recombinant Human TFRC Protein, His-tagged, Biotinylated | +Inquiry |
TFRC-296H | Recombinant Human TFRC Protein (ECD), His-tagged(C-ter) | +Inquiry |
TFRC-2179H | Recombinant Human TFRC Protein, His (Fc)-Avi-tagged | +Inquiry |
TFRC-137H | Recombinant Human TFRC Protein, C-His-tagged | +Inquiry |
◆ Native Protein | ||
TFRC-69H | Native Human Apotransferrin | +Inquiry |
TFRC-16H | Native Human Apotransferrin Protein | +Inquiry |
TFRC-249H | Native Human Transferrin Receptor | +Inquiry |
◆ Lysates | ||
TFRC-2058HCL | Recombinant Human TFRC cell lysate | +Inquiry |
TFRC-950CCL | Recombinant Cynomolgus TFRC cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionYes, TFRC's presence on cell surfaces has been exploited for the development of targeted drug delivery systems, especially for delivering drugs to cancer cells.
Yes, some experimental therapies aim to target TFRC on cancer cells, such as antibody-drug conjugates (ADCs) and small-molecule inhibitors.
Yes, dysregulation of TFRC expression or function can be associated with various medical conditions, including anemia, cancer, and neurodegenerative diseases.
Researchers use techniques like immunohistochemistry and molecular assays to study TFRC expression levels in patient samples. This helps in diagnosis and prognosis.
Understanding TFRC expression in the context of anemia can help identify the underlying causes and guide treatment strategies to improve iron uptake.
Customer Reviews (3)
Write a reviewIn addition to the advantages of GAD1 protein itself, the manufacturer can play a crucial role in supporting the research efforts.
This quality control is essential for obtaining reliable and reproducible data, as any variability in protein quality can introduce confounding factors into experiments.
This versatility enables researchers to explore GAD1's function and role in a range of biological processes, making it a valuable tool across diverse research fields.
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